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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia xenovorans Probable acetoacetate decarboxylase (adc) CSB-YP619865BNV
CSB-EP619865BNV
CSB-BP619865BNV
CSB-MP619865BNV
CSB-EP619865BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Dynactin subunit 1 (DCTN1), partial CSB-YP619866HU
CSB-BP619866HU
CSB-MP619866HU
CSB-EP619866HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription elongation factor B polypeptide 3 (TCEB3) CSB-YP619867HU
CSB-EP619867HU
CSB-BP619867HU
CSB-MP619867HU
CSB-EP619867HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Flotillin-2 (FLOT2) CSB-YP619868HU
CSB-BP619868HU
CSB-MP619868HU
CSB-EP619868HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP619869BNV
CSB-EP619869BNV
CSB-BP619869BNV
CSB-MP619869BNV
CSB-EP619869BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP619870BNV1
CSB-EP619870BNV1
CSB-BP619870BNV1
CSB-MP619870BNV1
CSB-EP619870BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Growth arrest-specific protein 6 (GAS6) CSB-YP619872HU
CSB-EP619872HU
CSB-BP619872HU
CSB-MP619872HU
CSB-EP619872HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Pantothenate synthetase (panC) CSB-YP619873BNV
CSB-EP619873BNV
CSB-BP619873BNV
CSB-MP619873BNV
CSB-EP619873BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Probable cytosol aminopeptidase (pepA), partial CSB-YP619874BNV
CSB-EP619874BNV
CSB-BP619874BNV
CSB-MP619874BNV
CSB-EP619874BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RNA-binding protein 39 (RBM39) CSB-YP619875HU
CSB-EP619875HU
CSB-BP619875HU
CSB-MP619875HU
CSB-EP619875HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Urease accessory protein UreE (ureE) CSB-YP619876BNV
CSB-EP619876BNV
CSB-BP619876BNV
CSB-MP619876BNV
CSB-EP619876BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human ATP-sensitive inward rectifier potassium channel 12 (KCNJ12), partial CSB-YP619877HU1
CSB-EP619877HU1
CSB-BP619877HU1
CSB-MP619877HU1
CSB-EP619877HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Keratin, type I cuticular Ha2 (KRT32) CSB-YP619878HU
CSB-BP619878HU
CSB-MP619878HU
CSB-EP619878HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human ATP-dependent RNA helicase DHX8 (DHX8), partial CSB-YP619879HU
CSB-EP619879HU
CSB-BP619879HU
CSB-MP619879HU
CSB-EP619879HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Protein TolB (tolB) CSB-YP619880BNV
CSB-EP619880BNV
CSB-BP619880BNV
CSB-MP619880BNV
CSB-EP619880BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Chaperone protein DnaJ (dnaJ) CSB-YP619881BNV
CSB-EP619881BNV
CSB-BP619881BNV
CSB-MP619881BNV
CSB-EP619881BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ferrochelatase (hemH) CSB-YP619882BNV
CSB-EP619882BNV
CSB-BP619882BNV
CSB-MP619882BNV
CSB-EP619882BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP619883BNV1
CSB-EP619883BNV1
CSB-BP619883BNV1
CSB-MP619883BNV1
CSB-EP619883BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP619884BNV
CSB-EP619884BNV
CSB-BP619884BNV
CSB-MP619884BNV
CSB-EP619884BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ras GTPase-activating protein 3 (RASA3), partial CSB-YP619885HU
CSB-EP619885HU
CSB-BP619885HU
CSB-MP619885HU
CSB-EP619885HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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