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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe DNA polymerase zeta processivity subunit (rev7) CSB-YP604943SXV
CSB-EP604943SXV
CSB-BP604943SXV
CSB-MP604943SXV
CSB-EP604943SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera 50S ribosomal protein L23, chloroplastic (rpl23-A) CSB-YP604944LGR
CSB-EP604944LGR
CSB-BP604944LGR
CSB-MP604944LGR
CSB-EP604944LGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP604945LGR
CSB-EP604945LGR
CSB-BP604945LGR
CSB-MP604945LGR
CSB-EP604945LGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera 50S ribosomal protein L2-B, chloroplastic (rpl2-B) CSB-YP604946LGR
CSB-EP604946LGR
CSB-BP604946LGR
CSB-MP604946LGR
CSB-EP604946LGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP604947LGR
CSB-EP604947LGR
CSB-BP604947LGR
CSB-MP604947LGR
CSB-EP604947LGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP604948LGR
CSB-EP604948LGR
CSB-BP604948LGR
CSB-MP604948LGR
CSB-EP604948LGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera Apocytochrome f (petA), partial CSB-YP604949LGR1
CSB-EP604949LGR1
CSB-BP604949LGR1
CSB-MP604949LGR1
CSB-EP604949LGR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera NAD (P)H-quinone oxidoreductase subunit J, chloroplastic (ndhJ) CSB-YP604950LGR
CSB-EP604950LGR
CSB-BP604950LGR
CSB-MP604950LGR
CSB-EP604950LGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera Photosystem II reaction center protein M (psbM), partial CSB-YP604951LGR
CSB-EP604951LGR
CSB-BP604951LGR
CSB-MP604951LGR
CSB-EP604951LGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriodendron tulipifera ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP604952LGR1
CSB-EP604952LGR1
CSB-BP604952LGR1
CSB-MP604952LGR1
CSB-EP604952LGR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Protein ycf2 (ycf2-A), partial CSB-YP604953DIR
CSB-EP604953DIR
CSB-BP604953DIR
CSB-MP604953DIR
CSB-EP604953DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic (ndhE), partial CSB-YP604954DIR1
CSB-EP604954DIR1
CSB-BP604954DIR1
CSB-MP604954DIR1
CSB-EP604954DIR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota 30S ribosomal protein S3, chloroplastic (rps3) CSB-YP604955DIR
CSB-EP604955DIR
CSB-BP604955DIR
CSB-MP604955DIR
CSB-EP604955DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP604956DIR1
CSB-EP604956DIR1
CSB-BP604956DIR1
CSB-MP604956DIR1
CSB-EP604956DIR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP604957DIR
CSB-EP604957DIR
CSB-BP604957DIR
CSB-MP604957DIR
CSB-EP604957DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Photosystem II reaction center protein L (psbL), partial CSB-YP604958DIR
CSB-EP604958DIR
CSB-BP604958DIR
CSB-MP604958DIR
CSB-EP604958DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota ATP synthase epsilon chain, chloroplastic (atpE) CSB-YP604959DIR
CSB-EP604959DIR
CSB-BP604959DIR
CSB-MP604959DIR
CSB-EP604959DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP604960DIR
CSB-EP604960DIR
CSB-BP604960DIR
CSB-MP604960DIR
CSB-EP604960DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota DNA-directed RNA polymerase subunit beta' (rpoC1), partial CSB-YP604961DIR
CSB-EP604961DIR
CSB-BP604961DIR
CSB-MP604961DIR
CSB-EP604961DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota 30S ribosomal protein S16, chloroplastic (rps16) CSB-YP604962DIR
CSB-EP604962DIR
CSB-BP604962DIR
CSB-MP604962DIR
CSB-EP604962DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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