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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Streptomyces griseus subsp. griseus Imidazolonepropionase (hutI) CSB-YP540258FOF
CSB-EP540258FOF
CSB-BP540258FOF
CSB-MP540258FOF
CSB-EP540258FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Peptide chain release factor 2 (prfB) CSB-YP540259FOF
CSB-EP540259FOF
CSB-BP540259FOF
CSB-MP540259FOF
CSB-EP540259FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP540260FOF
CSB-EP540260FOF
CSB-BP540260FOF
CSB-MP540260FOF
CSB-EP540260FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP540261FOF
CSB-EP540261FOF
CSB-BP540261FOF
CSB-MP540261FOF
CSB-EP540261FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable rRNA maturation factor (SGR_5003) CSB-YP540262FOF
CSB-EP540262FOF
CSB-BP540262FOF
CSB-MP540262FOF
CSB-EP540262FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP540263FOF
CSB-EP540263FOF
CSB-BP540263FOF
CSB-MP540263FOF
CSB-EP540263FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP540264FOF
CSB-EP540264FOF
CSB-BP540264FOF
CSB-MP540264FOF
CSB-EP540264FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable cytosol aminopeptidase (pepA), partial CSB-YP540265FOF
CSB-EP540265FOF
CSB-BP540265FOF
CSB-MP540265FOF
CSB-EP540265FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus UPF0286 protein SGR_2149 (SGR_2149) CSB-YP540266FOF
CSB-EP540266FOF
CSB-BP540266FOF
CSB-MP540266FOF
CSB-EP540266FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Tryptophan synthase beta chain (trpB) CSB-YP540267FOF
CSB-EP540267FOF
CSB-BP540267FOF
CSB-MP540267FOF
CSB-EP540267FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable 5-dehydro-4-deoxyglucarate dehydratase (SGR_5617) CSB-YP540268FOF
CSB-EP540268FOF
CSB-BP540268FOF
CSB-MP540268FOF
CSB-EP540268FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP540269FOF
CSB-EP540269FOF
CSB-BP540269FOF
CSB-MP540269FOF
CSB-EP540269FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Proteasome-associated ATPase (arc), partial CSB-YP540270FOF
CSB-EP540270FOF
CSB-BP540270FOF
CSB-MP540270FOF
CSB-EP540270FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP540271FOF
CSB-EP540271FOF
CSB-BP540271FOF
CSB-MP540271FOF
CSB-EP540271FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Probable transcriptional regulatory protein SGR_6014 (SGR_6014) CSB-YP540272FOF
CSB-EP540272FOF
CSB-BP540272FOF
CSB-MP540272FOF
CSB-EP540272FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP540273FOF
CSB-EP540273FOF
CSB-BP540273FOF
CSB-MP540273FOF
CSB-EP540273FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L13 (rplM) CSB-YP540274FOF
CSB-EP540274FOF
CSB-BP540274FOF
CSB-MP540274FOF
CSB-EP540274FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L17 (rplQ) CSB-YP540275FOF
CSB-EP540275FOF
CSB-BP540275FOF
CSB-MP540275FOF
CSB-EP540275FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L15 (rplO) CSB-YP540276FOF
CSB-EP540276FOF
CSB-BP540276FOF
CSB-MP540276FOF
CSB-EP540276FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus 50S ribosomal protein L14 (rplN) CSB-YP540277FOF
CSB-EP540277FOF
CSB-BP540277FOF
CSB-MP540277FOF
CSB-EP540277FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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