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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Ktr system potassium uptake protein D (ktrD), partial CSB-YP521746BRJ
CSB-EP521746BRJ
CSB-BP521746BRJ
CSB-MP521746BRJ
CSB-EP521746BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0012 hydrolase mtnU (mtnU) CSB-YP521747BRJ
CSB-EP521747BRJ
CSB-BP521747BRJ
CSB-MP521747BRJ
CSB-EP521747BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ykvA (ykvA), partial CSB-YP521748BRJ1
CSB-EP521748BRJ1
CSB-BP521748BRJ1
CSB-MP521748BRJ1
CSB-EP521748BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP-dependent Clp protease ATP-binding subunit ClpE (clpE), partial CSB-YP521749BRJ
CSB-EP521749BRJ
CSB-BP521749BRJ
CSB-MP521749BRJ
CSB-EP521749BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP521750BRJ
CSB-EP521750BRJ
CSB-BP521750BRJ
CSB-MP521750BRJ
CSB-EP521750BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative glycosylase ykvQ (ykvQ) CSB-YP521751BRJ
CSB-EP521751BRJ
CSB-BP521751BRJ
CSB-MP521751BRJ
CSB-EP521751BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation thiol-disulfide oxidoreductase A (stoA) CSB-YP521752BRJ
CSB-EP521752BRJ
CSB-BP521752BRJ
CSB-MP521752BRJ
CSB-EP521752BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter ykuC (ykuC), partial CSB-YP521753BRJ
CSB-EP521753BRJ
CSB-BP521753BRJ
CSB-MP521753BRJ
CSB-EP521753BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thiol-disulfide oxidoreductase ykuV (ykuV) CSB-YP521754BRJ
CSB-EP521754BRJ
CSB-BP521754BRJ
CSB-MP521754BRJ
CSB-EP521754BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Molybdopterin molybdenumtransferase (moeA) CSB-YP521755BRJ
CSB-EP521755BRJ
CSB-BP521755BRJ
CSB-MP521755BRJ
CSB-EP521755BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YknU (yknU), partial CSB-YP521756BRJ1
CSB-EP521756BRJ1
CSB-BP521756BRJ1
CSB-MP521756BRJ1
CSB-EP521756BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter permease yknZ (yknZ), partial CSB-YP521757BRJ1
CSB-EP521757BRJ1
CSB-BP521757BRJ1
CSB-MP521757BRJ1
CSB-EP521757BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase ykpB (ykpB) CSB-YP521758BRJ
CSB-EP521758BRJ
CSB-BP521758BRJ
CSB-MP521758BRJ
CSB-EP521758BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase (ylmB) CSB-YP521759BRJ
CSB-EP521759BRJ
CSB-BP521759BRJ
CSB-MP521759BRJ
CSB-EP521759BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell division protein SepF (sepF) CSB-YP521760BRJ
CSB-EP521760BRJ
CSB-BP521760BRJ
CSB-MP521760BRJ
CSB-EP521760BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribosome maturation factor RimM (rimM) CSB-YP521761BRJ
CSB-EP521761BRJ
CSB-BP521761BRJ
CSB-MP521761BRJ
CSB-EP521761BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ylqG (ylqG), partial CSB-YP521762BRJ
CSB-EP521762BRJ
CSB-BP521762BRJ
CSB-MP521762BRJ
CSB-EP521762BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Zinc metalloprotease rasP (rasP), partial CSB-YP521763BRJ1
CSB-EP521763BRJ1
CSB-BP521763BRJ1
CSB-MP521763BRJ1
CSB-EP521763BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable inactive metalloprotease ymfF (ymfF) CSB-YP521764BRJ
CSB-EP521764BRJ
CSB-BP521764BRJ
CSB-MP521764BRJ
CSB-EP521764BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ymfM (ymfM), partial CSB-YP521765BRJ1
CSB-EP521765BRJ1
CSB-BP521765BRJ1
CSB-MP521765BRJ1
CSB-EP521765BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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