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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Altronate hydrolase (uxaA) CSB-YP520245BRJ
CSB-EP520245BRJ
CSB-BP520245BRJ
CSB-MP520245BRJ
CSB-EP520245BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjiA (yjiA) CSB-YP520246BRJ
CSB-EP520246BRJ
CSB-BP520246BRJ
CSB-MP520246BRJ
CSB-EP520246BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0014 membrane protein yjkA (yjkA), partial CSB-YP520247BRJ1
CSB-EP520247BRJ1
CSB-BP520247BRJ1
CSB-MP520247BRJ1
CSB-EP520247BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ring-cleaving dioxygenase mhqA (mhqA) CSB-YP520248BRJ
CSB-EP520248BRJ
CSB-BP520248BRJ
CSB-MP520248BRJ
CSB-EP520248BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transferase ytoA (ytoA) CSB-YP520249BRJ
CSB-EP520249BRJ
CSB-BP520249BRJ
CSB-MP520249BRJ
CSB-EP520249BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yotC (yotC) CSB-YP520250BRJ
CSB-EP520250BRJ
CSB-BP520250BRJ
CSB-MP520250BRJ
CSB-EP520250BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phospholipase ytpA (ytpA) CSB-YP520251BRJ
CSB-EP520251BRJ
CSB-BP520251BRJ
CSB-MP520251BRJ
CSB-EP520251BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional repressor ytrA (ytrA) CSB-YP520252BRJ
CSB-EP520252BRJ
CSB-BP520252BRJ
CSB-MP520252BRJ
CSB-EP520252BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Major myo-inositol transporter iolT (iolT), partial CSB-YP520253BRJ
CSB-EP520253BRJ
CSB-BP520253BRJ
CSB-MP520253BRJ
CSB-EP520253BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein yjhA (yjhA) CSB-YP520254BRJ
CSB-EP520254BRJ
CSB-BP520254BRJ
CSB-MP520254BRJ
CSB-EP520254BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized NTE family protein ylbK (ylbK) CSB-YP520255BRJ
CSB-EP520255BRJ
CSB-BP520255BRJ
CSB-MP520255BRJ
CSB-EP520255BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvpB (yvpB) CSB-YP520256BRJ
CSB-EP520256BRJ
CSB-BP520256BRJ
CSB-MP520256BRJ
CSB-EP520256BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Serine/threonine exchanger SteT (steT), partial CSB-YP520257BRJ
CSB-EP520257BRJ
CSB-BP520257BRJ
CSB-MP520257BRJ
CSB-EP520257BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized symporter yodF (yodF), partial CSB-YP520258BRJ
CSB-EP520258BRJ
CSB-BP520258BRJ
CSB-MP520258BRJ
CSB-EP520258BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (tagO), partial CSB-YP520259BRJ
CSB-EP520259BRJ
CSB-BP520259BRJ
CSB-MP520259BRJ
CSB-EP520259BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Organic hydroperoxide resistance protein ohrA (ohrA) CSB-YP520260BRJ
CSB-EP520260BRJ
CSB-BP520260BRJ
CSB-MP520260BRJ
CSB-EP520260BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Oxalate decarboxylase oxdD (oxdD) CSB-YP520261BRJ
CSB-EP520261BRJ
CSB-BP520261BRJ
CSB-MP520261BRJ
CSB-EP520261BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived putative transcriptional regulator yosT (yosT) CSB-YP520262BRJ
CSB-EP520262BRJ
CSB-BP520262BRJ
CSB-MP520262BRJ
CSB-EP520262BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobF (yobF) CSB-YP520263BRJ
CSB-EP520263BRJ
CSB-BP520263BRJ
CSB-MP520263BRJ
CSB-EP520263BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Polyketide biosynthesis protein pksE (pksE), partial CSB-YP520264BRJ
CSB-EP520264BRJ
CSB-BP520264BRJ
CSB-MP520264BRJ
CSB-EP520264BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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