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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP518503BRJ
CSB-EP518503BRJ
CSB-BP518503BRJ
CSB-MP518503BRJ
CSB-EP518503BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA polymerase III subunit alpha (dnaE), partial CSB-YP518504BRJ
CSB-EP518504BRJ
CSB-BP518504BRJ
CSB-MP518504BRJ
CSB-EP518504BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein yerH (yerH) CSB-YP518505BRJ
CSB-EP518505BRJ
CSB-BP518505BRJ
CSB-MP518505BRJ
CSB-EP518505BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable L-serine dehydratase, beta chain (sdaAB) CSB-YP518506BRJ
CSB-EP518506BRJ
CSB-BP518506BRJ
CSB-MP518506BRJ
CSB-EP518506BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YerI (yerI) CSB-YP518507BRJ
CSB-EP518507BRJ
CSB-BP518507BRJ
CSB-MP518507BRJ
CSB-EP518507BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yobD (yobD) CSB-YP518508BRJ
CSB-EP518508BRJ
CSB-BP518508BRJ
CSB-MP518508BRJ
CSB-EP518508BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yodI (yodI), partial CSB-YP518509BRJ1
CSB-EP518509BRJ1
CSB-BP518509BRJ1
CSB-MP518509BRJ1
CSB-EP518509BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable 2-dehydropantoate 2-reductase (panE) CSB-YP518510BRJ
CSB-EP518510BRJ
CSB-BP518510BRJ
CSB-MP518510BRJ
CSB-EP518510BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis S-ribosylhomocysteine lyase (luxS) CSB-YP518511BRJ
CSB-BP518511BRJ
CSB-MP518511BRJ
CSB-EP518511BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable cell division protein ytgP (ytgP), partial CSB-YP518512BRJ
CSB-EP518512BRJ
CSB-BP518512BRJ
CSB-MP518512BRJ
CSB-EP518512BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable BsuMI modification methylase subunit ydiP (ydiP) CSB-YP518513BRJ
CSB-EP518513BRJ
CSB-BP518513BRJ
CSB-MP518513BRJ
CSB-EP518513BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator YofA (yofA) CSB-YP518514BRJ
CSB-EP518514BRJ
CSB-BP518514BRJ
CSB-MP518514BRJ
CSB-EP518514BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative niacin/nicotinamide transporter NaiP (naiP), partial CSB-YP518515BRJ
CSB-EP518515BRJ
CSB-BP518515BRJ
CSB-MP518515BRJ
CSB-EP518515BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bacitracin export ATP-binding protein BceA (bceA) CSB-YP518516BRJ
CSB-EP518516BRJ
CSB-BP518516BRJ
CSB-MP518516BRJ
CSB-EP518516BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ATPase YjoB (yjoB) CSB-YP518517BRJ
CSB-EP518517BRJ
CSB-BP518517BRJ
CSB-MP518517BRJ
CSB-EP518517BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ABC transporter permease protein AmyD (amyD), partial CSB-YP518518BRJ1
CSB-EP518518BRJ1
CSB-BP518518BRJ1
CSB-MP518518BRJ1
CSB-EP518518BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Oxalate decarboxylase oxdC (oxdC) CSB-YP518519BRJ
CSB-EP518519BRJ
CSB-BP518519BRJ
CSB-MP518519BRJ
CSB-EP518519BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfmG (yfmG) CSB-YP518520BRJ
CSB-EP518520BRJ
CSB-BP518520BRJ
CSB-MP518520BRJ
CSB-EP518520BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP518521BRJ
CSB-EP518521BRJ
CSB-BP518521BRJ
CSB-MP518521BRJ
CSB-EP518521BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yjmC (yjmC) CSB-YP518522BRJ
CSB-EP518522BRJ
CSB-BP518522BRJ
CSB-MP518522BRJ
CSB-EP518522BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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