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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein yhaI (yhaI) CSB-YP517003BRJ
CSB-EP517003BRJ
CSB-BP517003BRJ
CSB-MP517003BRJ
CSB-EP517003BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor histidine kinase yhcY (yhcY) CSB-YP517004BRJ
CSB-EP517004BRJ
CSB-BP517004BRJ
CSB-MP517004BRJ
CSB-EP517004BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative enoyl-CoA hydratase/isomerase yhaR (yhaR), partial CSB-YP517005BRJ1
CSB-EP517005BRJ1
CSB-BP517005BRJ1
CSB-MP517005BRJ1
CSB-EP517005BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0342 protein yheA (yheA) CSB-YP517006BRJ
CSB-EP517006BRJ
CSB-BP517006BRJ
CSB-MP517006BRJ
CSB-EP517006BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yheG (yheG) CSB-YP517007BRJ
CSB-EP517007BRJ
CSB-BP517007BRJ
CSB-MP517007BRJ
CSB-EP517007BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhjD (yhjD) CSB-YP517008BRJ
CSB-EP517008BRJ
CSB-BP517008BRJ
CSB-MP517008BRJ
CSB-EP517008BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis NTD biosynthesis operon putative oxidoreductase NtdC (ntdC) CSB-YP517009BRJ
CSB-EP517009BRJ
CSB-BP517009BRJ
CSB-MP517009BRJ
CSB-EP517009BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhjN (yhjN), partial CSB-YP517010BRJ
CSB-EP517010BRJ
CSB-BP517010BRJ
CSB-MP517010BRJ
CSB-EP517010BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YhdJ (yhdJ) CSB-YP517011BRJ
CSB-EP517011BRJ
CSB-BP517011BRJ
CSB-MP517011BRJ
CSB-EP517011BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 1-acyl-sn-glycerol-3-phosphate acyltransferase (plsC) CSB-YP517012BRJ
CSB-EP517012BRJ
CSB-BP517012BRJ
CSB-MP517012BRJ
CSB-EP517012BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein CrcB homolog 1 (crcB1), partial CSB-YP517013BRJ1
CSB-EP517013BRJ1
CSB-BP517013BRJ1
CSB-MP517013BRJ1
CSB-EP517013BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative amidohydrolase yhaA (yhaA) CSB-YP517014BRJ
CSB-EP517014BRJ
CSB-BP517014BRJ
CSB-MP517014BRJ
CSB-EP517014BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhfH (yhfH) CSB-YP517015BRJ
CSB-EP517015BRJ
CSB-BP517015BRJ
CSB-MP517015BRJ
CSB-EP517015BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized sugar epimerase yhfK (yhfK) CSB-YP517016BRJ
CSB-EP517016BRJ
CSB-BP517016BRJ
CSB-MP517016BRJ
CSB-EP517016BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized phosphatase phoE (phoE) CSB-YP517017BRJ
CSB-EP517017BRJ
CSB-BP517017BRJ
CSB-MP517017BRJ
CSB-EP517017BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis RNA polymerase sigma factor ylaC (ylaC) CSB-YP517018BRJ
CSB-EP517018BRJ
CSB-BP517018BRJ
CSB-MP517018BRJ
CSB-EP517018BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis GTP-binding protein TypA/BipA homolog (typA), partial CSB-YP517019BRJ
CSB-EP517019BRJ
CSB-BP517019BRJ
CSB-MP517019BRJ
CSB-EP517019BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0358 protein ylaN (ylaN) CSB-YP517020BRJ
CSB-EP517020BRJ
CSB-BP517020BRJ
CSB-MP517020BRJ
CSB-EP517020BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis HPr kinase/phosphorylase (hprK) CSB-YP517021ELW
CSB-EP517021ELW
CSB-BP517021ELW
CSB-MP517021ELW
CSB-EP517021ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosomal protein L11 methyltransferase (prmA) CSB-YP517022HUV
CSB-EP517022HUV
CSB-BP517022HUV
CSB-MP517022HUV
CSB-EP517022HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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