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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis UPF0001 protein ylmE (ylmE) CSB-YP516301BRJ
CSB-EP516301BRJ
CSB-BP516301BRJ
CSB-MP516301BRJ
CSB-EP516301BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0109 protein ylqC (ylqC) CSB-YP516302BRJ
CSB-EP516302BRJ
CSB-BP516302BRJ
CSB-MP516302BRJ
CSB-EP516302BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribosome biogenesis GTPase A (rbgA) CSB-YP516303BRJ
CSB-EP516303BRJ
CSB-BP516303BRJ
CSB-MP516303BRJ
CSB-EP516303BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP516304BRJ
CSB-EP516304BRJ
CSB-BP516304BRJ
CSB-MP516304BRJ
CSB-EP516304BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bacillibactin exporter (ymfD), partial CSB-YP516305BRJ
CSB-EP516305BRJ
CSB-BP516305BRJ
CSB-MP516305BRJ
CSB-EP516305BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ymfK (ymfK) CSB-YP516306BRJ
CSB-EP516306BRJ
CSB-BP516306BRJ
CSB-MP516306BRJ
CSB-EP516306BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ymcC (ymcC), partial CSB-YP516307BRJ1
CSB-EP516307BRJ1
CSB-BP516307BRJ1
CSB-MP516307BRJ1
CSB-EP516307BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ymaE (ymaE) CSB-YP516308BRJ
CSB-EP516308BRJ
CSB-BP516308BRJ
CSB-MP516308BRJ
CSB-EP516308BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ymaG (ymaG), partial CSB-YP516309BRJ1
CSB-EP516309BRJ1
CSB-BP516309BRJ1
CSB-MP516309BRJ1
CSB-EP516309BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ymzC (ymzC) CSB-YP516310BRJ
CSB-EP516310BRJ
CSB-BP516310BRJ
CSB-MP516310BRJ
CSB-EP516310BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ynzH (ynzH) CSB-YP516311BRJ
CSB-EP516311BRJ
CSB-BP516311BRJ
CSB-MP516311BRJ
CSB-EP516311BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ynzB (ynzB) CSB-YP516312BRJ
CSB-EP516312BRJ
CSB-BP516312BRJ
CSB-MP516312BRJ
CSB-EP516312BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore germination protein YndE (yndE), partial CSB-YP516313BRJ
CSB-EP516313BRJ
CSB-BP516313BRJ
CSB-MP516313BRJ
CSB-EP516313BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0714 protein yndL (yndL), partial CSB-YP516314BRJ1
CSB-EP516314BRJ1
CSB-BP516314BRJ1
CSB-MP516314BRJ1
CSB-EP516314BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aspartyl-phosphate phosphatase ynzD (ynzD) CSB-YP516315BRJ
CSB-EP516315BRJ
CSB-BP516315BRJ
CSB-MP516315BRJ
CSB-EP516315BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Oligoribonuclease nrnB (nrnB) CSB-YP516316BRJ
CSB-EP516316BRJ
CSB-BP516316BRJ
CSB-MP516316BRJ
CSB-EP516316BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yoaQ (yoaQ) CSB-YP516317BRJ
CSB-EP516317BRJ
CSB-BP516317BRJ
CSB-MP516317BRJ
CSB-EP516317BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobA (yobA) CSB-YP516318BRJ
CSB-EP516318BRJ
CSB-BP516318BRJ
CSB-MP516318BRJ
CSB-EP516318BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yozL (yozL) CSB-YP516319BRJ
CSB-EP516319BRJ
CSB-BP516319BRJ
CSB-MP516319BRJ
CSB-EP516319BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yozO (yozO) CSB-YP516320BRJ
CSB-EP516320BRJ
CSB-BP516320BRJ
CSB-MP516320BRJ
CSB-EP516320BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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