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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella melitensis biotype 2 Proline--tRNA ligase (proS) CSB-YP507962BWT
CSB-EP507962BWT
CSB-BP507962BWT
CSB-MP507962BWT
CSB-EP507962BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-dehydroquinate dehydratase (aroQ) CSB-YP507963BWT
CSB-EP507963BWT
CSB-BP507963BWT
CSB-MP507963BWT
CSB-EP507963BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP507964BWT
CSB-EP507964BWT
CSB-BP507964BWT
CSB-MP507964BWT
CSB-EP507964BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Enolase (eno) CSB-YP507965BWT
CSB-EP507965BWT
CSB-BP507965BWT
CSB-MP507965BWT
CSB-EP507965BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP507966BWT
CSB-EP507966BWT
CSB-BP507966BWT
CSB-MP507966BWT
CSB-EP507966BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L17 (rplQ) CSB-YP507967BWT
CSB-EP507967BWT
CSB-BP507967BWT
CSB-MP507967BWT
CSB-EP507967BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L30 (rpmD) CSB-YP507968BWT
CSB-EP507968BWT
CSB-BP507968BWT
CSB-MP507968BWT
CSB-EP507968BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L5 (rplE) CSB-YP507969BWT
CSB-EP507969BWT
CSB-BP507969BWT
CSB-MP507969BWT
CSB-EP507969BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S19 (rpsS) CSB-YP507970BWT
CSB-EP507970BWT
CSB-BP507970BWT
CSB-MP507970BWT
CSB-EP507970BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S12 (rpsL) CSB-YP507971BWT
CSB-EP507971BWT
CSB-BP507971BWT
CSB-MP507971BWT
CSB-EP507971BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Sugar fermentation stimulation protein homolog (sfsA) CSB-YP507972BWT
CSB-EP507972BWT
CSB-BP507972BWT
CSB-MP507972BWT
CSB-EP507972BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP507973BWT
CSB-EP507973BWT
CSB-BP507973BWT
CSB-MP507973BWT
CSB-EP507973BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Protein nrdI (nrdI) CSB-YP507974BWT
CSB-EP507974BWT
CSB-BP507974BWT
CSB-MP507974BWT
CSB-EP507974BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Biotin synthase (bioB) CSB-YP507975BWT
CSB-EP507975BWT
CSB-BP507975BWT
CSB-MP507975BWT
CSB-EP507975BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Aspartate carbamoyltransferase (pyrB) CSB-YP507976BWT
CSB-EP507976BWT
CSB-BP507976BWT
CSB-MP507976BWT
CSB-EP507976BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP507977BWT
CSB-EP507977BWT
CSB-BP507977BWT
CSB-MP507977BWT
CSB-EP507977BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 D-aminopeptidase (dap), partial CSB-YP507978BWT
CSB-EP507978BWT
CSB-BP507978BWT
CSB-MP507978BWT
CSB-EP507978BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Methylglyoxal synthase (mgsA) CSB-YP507979BWT
CSB-EP507979BWT
CSB-BP507979BWT
CSB-MP507979BWT
CSB-EP507979BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Altered inheritance of mitochondria protein 31, mitochondrial (AIM31), partial CSB-YP507980ERP1
CSB-EP507980ERP1
CSB-BP507980ERP1
CSB-MP507980ERP1
CSB-EP507980ERP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis 40S ribosomal protein S0 (RPS0) CSB-YP507981ERP
CSB-EP507981ERP
CSB-BP507981ERP
CSB-MP507981ERP
CSB-EP507981ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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