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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella melitensis biotype 2 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP507282BWT
CSB-EP507282BWT
CSB-BP507282BWT
CSB-MP507282BWT
CSB-EP507282BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP507283BWT
CSB-EP507283BWT
CSB-BP507283BWT
CSB-MP507283BWT
CSB-EP507283BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribosome maturation factor RimP (rimP) CSB-YP507284BWT
CSB-EP507284BWT
CSB-BP507284BWT
CSB-MP507284BWT
CSB-EP507284BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Cytidylate kinase (cmk) CSB-YP507285BWT
CSB-EP507285BWT
CSB-BP507285BWT
CSB-MP507285BWT
CSB-EP507285BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Putative pterin-4-alpha-carbinolamine dehydratase (BMEA_A0085) CSB-YP507286BWT
CSB-EP507286BWT
CSB-BP507286BWT
CSB-MP507286BWT
CSB-EP507286BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribonuclease PH (rph) CSB-YP507287BWT
CSB-EP507287BWT
CSB-BP507287BWT
CSB-MP507287BWT
CSB-EP507287BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribonuclease HII (rnhB) CSB-YP507288BWT
CSB-EP507288BWT
CSB-BP507288BWT
CSB-MP507288BWT
CSB-EP507288BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S18 (rpsR) CSB-YP507289BWT
CSB-EP507289BWT
CSB-BP507289BWT
CSB-MP507289BWT
CSB-EP507289BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ureidoglycolate hydrolase (allA) CSB-YP507290BWT
CSB-EP507290BWT
CSB-BP507290BWT
CSB-MP507290BWT
CSB-EP507290BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP507291BWT
CSB-EP507291BWT
CSB-BP507291BWT
CSB-MP507291BWT
CSB-EP507291BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP507292BWT
CSB-EP507292BWT
CSB-BP507292BWT
CSB-MP507292BWT
CSB-EP507292BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L13 (rplM) CSB-YP507293BWT
CSB-EP507293BWT
CSB-BP507293BWT
CSB-MP507293BWT
CSB-EP507293BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S4 (rpsD) CSB-YP507294BWT
CSB-EP507294BWT
CSB-BP507294BWT
CSB-MP507294BWT
CSB-EP507294BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Sec-independent protein translocase protein TatA (tatA), partial CSB-YP507295BWT
CSB-EP507295BWT
CSB-BP507295BWT
CSB-MP507295BWT
CSB-EP507295BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP507296BWT
CSB-EP507296BWT
CSB-BP507296BWT
CSB-MP507296BWT
CSB-EP507296BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Phosphopantetheine adenylyltransferase (coaD) CSB-YP507297BWT
CSB-EP507297BWT
CSB-BP507297BWT
CSB-MP507297BWT
CSB-EP507297BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP507298BWT
CSB-EP507298BWT
CSB-BP507298BWT
CSB-MP507298BWT
CSB-EP507298BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S5 (rpsE) CSB-YP507299BWT
CSB-EP507299BWT
CSB-BP507299BWT
CSB-MP507299BWT
CSB-EP507299BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L14 (rplN) CSB-YP507300BWT
CSB-EP507300BWT
CSB-BP507300BWT
CSB-MP507300BWT
CSB-EP507300BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L3 (rplC) CSB-YP507301BWT
CSB-EP507301BWT
CSB-BP507301BWT
CSB-MP507301BWT
CSB-EP507301BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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