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Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis 7-cyano-7-deazaguanine synthase (queC) CSB-YP503342BQF
CSB-EP503342BQF
CSB-BP503342BQF
CSB-MP503342BQF
CSB-EP503342BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP503343BQF
CSB-EP503343BQF
CSB-BP503343BQF
CSB-MP503343BQF
CSB-EP503343BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP503344BQF
CSB-EP503344BQF
CSB-BP503344BQF
CSB-MP503344BQF
CSB-EP503344BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis D-alanine--poly (phosphoribitol) ligase subunit 1, partial CSB-YP503345BQF
CSB-EP503345BQF
CSB-BP503345BQF
CSB-MP503345BQF
CSB-EP503345BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazolonepropionase (hutI) CSB-YP503346BQF
CSB-EP503346BQF
CSB-BP503346BQF
CSB-MP503346BQF
CSB-EP503346BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Hut operon positive regulatory protein (hutP) CSB-YP503347BQF
CSB-EP503347BQF
CSB-BP503347BQF
CSB-MP503347BQF
CSB-EP503347BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0154 protein BAA_3767 (BAA_3767), partial CSB-YP503348BQF
CSB-EP503348BQF
CSB-BP503348BQF
CSB-MP503348BQF
CSB-EP503348BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Protein hfq (hfq) CSB-YP503349BQF
CSB-EP503349BQF
CSB-BP503349BQF
CSB-MP503349BQF
CSB-EP503349BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0180 protein BAA_1480 (BAA_1480) CSB-YP503350BQF
CSB-EP503350BQF
CSB-BP503350BQF
CSB-MP503350BQF
CSB-EP503350BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 2-isopropylmalate synthase (leuA), partial CSB-YP503351BQF
CSB-EP503351BQF
CSB-BP503351BQF
CSB-MP503351BQF
CSB-EP503351BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP503352BQF
CSB-EP503352BQF
CSB-BP503352BQF
CSB-MP503352BQF
CSB-EP503352BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP503353BQF
CSB-EP503353BQF
CSB-BP503353BQF
CSB-MP503353BQF
CSB-EP503353BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP503354BQF
CSB-EP503354BQF
CSB-BP503354BQF
CSB-MP503354BQF
CSB-EP503354BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis ATP phosphoribosyltransferase (hisG) CSB-YP503355BQF
CSB-EP503355BQF
CSB-BP503355BQF
CSB-MP503355BQF
CSB-EP503355BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP503356BQF
CSB-EP503356BQF
CSB-BP503356BQF
CSB-MP503356BQF
CSB-EP503356BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP503357BQF
CSB-EP503357BQF
CSB-BP503357BQF
CSB-MP503357BQF
CSB-EP503357BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP503358BQF
CSB-EP503358BQF
CSB-BP503358BQF
CSB-MP503358BQF
CSB-EP503358BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP503359BQF
CSB-EP503359BQF
CSB-BP503359BQF
CSB-MP503359BQF
CSB-EP503359BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP503360BQF
CSB-EP503360BQF
CSB-BP503360BQF
CSB-MP503360BQF
CSB-EP503360BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP503361BQF
CSB-EP503361BQF
CSB-BP503361BQF
CSB-MP503361BQF
CSB-EP503361BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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