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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP501562BQG
CSB-EP501562BQG
CSB-BP501562BQG
CSB-MP501562BQG
CSB-EP501562BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0173 metal-dependent hydrolase BAMEG_4892 (BAMEG_4892) CSB-YP501563BQG
CSB-EP501563BQG
CSB-BP501563BQG
CSB-MP501563BQG
CSB-EP501563BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0154 protein BAMEG_0893 (BAMEG_0893), partial CSB-YP501564BQG
CSB-EP501564BQG
CSB-BP501564BQG
CSB-MP501564BQG
CSB-EP501564BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Hut operon positive regulatory protein (hutP) CSB-YP501565BQG
CSB-EP501565BQG
CSB-BP501565BQG
CSB-MP501565BQG
CSB-EP501565BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazolonepropionase (hutI) CSB-YP501566BQG
CSB-EP501566BQG
CSB-BP501566BQG
CSB-MP501566BQG
CSB-EP501566BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Small, acid-soluble spore protein O (sspO) CSB-YP501567BQG
CSB-EP501567BQG
CSB-BP501567BQG
CSB-MP501567BQG
CSB-EP501567BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Small, acid-soluble spore protein N (sspN) CSB-YP501568BQG
CSB-EP501568BQG
CSB-BP501568BQG
CSB-MP501568BQG
CSB-EP501568BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Small, acid-soluble spore protein tlp (tlp) CSB-YP501569BQG
CSB-EP501569BQG
CSB-BP501569BQG
CSB-MP501569BQG
CSB-EP501569BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP501570BQG
CSB-EP501570BQG
CSB-BP501570BQG
CSB-MP501570BQG
CSB-EP501570BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis GTPase Der (der) CSB-YP501571BQG
CSB-EP501571BQG
CSB-BP501571BQG
CSB-MP501571BQG
CSB-EP501571BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP501572BQG
CSB-EP501572BQG
CSB-BP501572BQG
CSB-MP501572BQG
CSB-EP501572BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Cytidylate kinase (cmk) CSB-YP501573BQG
CSB-EP501573BQG
CSB-BP501573BQG
CSB-MP501573BQG
CSB-EP501573BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP501574BQG
CSB-EP501574BQG
CSB-BP501574BQG
CSB-MP501574BQG
CSB-EP501574BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Sulfate adenylyltransferase (sat) CSB-YP501575BQG
CSB-EP501575BQG
CSB-BP501575BQG
CSB-MP501575BQG
CSB-EP501575BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP501576BQG
CSB-EP501576BQG
CSB-BP501576BQG
CSB-MP501576BQG
CSB-EP501576BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP501577BQG
CSB-EP501577BQG
CSB-BP501577BQG
CSB-MP501577BQG
CSB-EP501577BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP501578BQG
CSB-EP501578BQG
CSB-BP501578BQG
CSB-MP501578BQG
CSB-EP501578BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP501579BQG
CSB-EP501579BQG
CSB-BP501579BQG
CSB-MP501579BQG
CSB-EP501579BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP501580BQG
CSB-EP501580BQG
CSB-BP501580BQG
CSB-MP501580BQG
CSB-EP501580BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP501581BQG
CSB-EP501581BQG
CSB-BP501581BQG
CSB-MP501581BQG
CSB-EP501581BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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