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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP490980BWY
CSB-EP490980BWY
CSB-BP490980BWY
CSB-MP490980BWY
CSB-EP490980BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Phosphoheptose isomerase (gmhA) CSB-YP490981BWY
CSB-EP490981BWY
CSB-BP490981BWY
CSB-MP490981BWY
CSB-EP490981BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP490982BWY
CSB-EP490982BWY
CSB-BP490982BWY
CSB-MP490982BWY
CSB-EP490982BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP490983BWY
CSB-EP490983BWY
CSB-BP490983BWY
CSB-MP490983BWY
CSB-EP490983BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Probable septum site-determining protein MinC (minC) CSB-YP490984BWY
CSB-EP490984BWY
CSB-BP490984BWY
CSB-MP490984BWY
CSB-EP490984BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Probable cytosol aminopeptidase (pepA) CSB-YP490985BWY
CSB-EP490985BWY
CSB-BP490985BWY
CSB-MP490985BWY
CSB-EP490985BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 50S ribosomal protein L21 (rplU) CSB-YP490986BWY
CSB-EP490986BWY
CSB-BP490986BWY
CSB-MP490986BWY
CSB-EP490986BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 30S ribosomal protein S16 (rpsP) CSB-YP490987BWY
CSB-EP490987BWY
CSB-BP490987BWY
CSB-MP490987BWY
CSB-EP490987BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Enolase (eno) CSB-YP490988BWY
CSB-EP490988BWY
CSB-BP490988BWY
CSB-MP490988BWY
CSB-EP490988BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum UPF0133 protein BUAP5A_475 (BUAP5A_475) CSB-YP490989BWY
CSB-EP490989BWY
CSB-BP490989BWY
CSB-MP490989BWY
CSB-EP490989BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 30S ribosomal protein S11 (rpsK) CSB-YP490990BWY
CSB-EP490990BWY
CSB-BP490990BWY
CSB-MP490990BWY
CSB-EP490990BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 50S ribosomal protein L24 (rplX) CSB-YP490991BWY
CSB-EP490991BWY
CSB-BP490991BWY
CSB-MP490991BWY
CSB-EP490991BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 50S ribosomal protein L23 (rplW) CSB-YP490992BWY
CSB-EP490992BWY
CSB-BP490992BWY
CSB-MP490992BWY
CSB-EP490992BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Protein TusC (tusC) CSB-YP490993BWY
CSB-EP490993BWY
CSB-BP490993BWY
CSB-MP490993BWY
CSB-EP490993BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a Cobyric acid synthase (cobQ), partial CSB-YP490994LPW
CSB-EP490994LPW
CSB-BP490994LPW
CSB-MP490994LPW
CSB-EP490994LPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP490995LPW
CSB-EP490995LPW
CSB-BP490995LPW
CSB-MP490995LPW
CSB-EP490995LPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a 30S ribosomal protein S18 (rpsR) CSB-YP490996LPW
CSB-EP490996LPW
CSB-BP490996LPW
CSB-MP490996LPW
CSB-EP490996LPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a Potassium-transporting ATPase B chain (kdpB), partial CSB-YP490997LPW1
CSB-EP490997LPW1
CSB-BP490997LPW1
CSB-MP490997LPW1
CSB-EP490997LPW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a 50S ribosomal protein L4 (rplD) CSB-YP490998LPW
CSB-EP490998LPW
CSB-BP490998LPW
CSB-MP490998LPW
CSB-EP490998LPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a 50S ribosomal protein L5 (rplE) CSB-YP490999LPW
CSB-EP490999LPW
CSB-BP490999LPW
CSB-MP490999LPW
CSB-EP490999LPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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