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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia fergusonii NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP483277EOR
CSB-EP483277EOR
CSB-BP483277EOR
CSB-MP483277EOR
CSB-EP483277EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP483278EOR1
CSB-EP483278EOR1
CSB-BP483278EOR1
CSB-MP483278EOR1
CSB-EP483278EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii N utilization substance protein B homolog (nusB) CSB-YP483279EOR
CSB-EP483279EOR
CSB-BP483279EOR
CSB-MP483279EOR
CSB-EP483279EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Recombination-associated protein rdgC (rdgC) CSB-YP483280EOR
CSB-EP483280EOR
CSB-BP483280EOR
CSB-MP483280EOR
CSB-EP483280EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Aspartate carbamoyltransferase (pyrB) CSB-YP483281EOR
CSB-EP483281EOR
CSB-BP483281EOR
CSB-MP483281EOR
CSB-EP483281EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP483282EOR
CSB-EP483282EOR
CSB-BP483282EOR
CSB-MP483282EOR
CSB-EP483282EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Hydroxylamine reductase (hcp), partial CSB-YP483283EOR
CSB-EP483283EOR
CSB-BP483283EOR
CSB-MP483283EOR
CSB-EP483283EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP483284EOR
CSB-EP483284EOR
CSB-BP483284EOR
CSB-MP483284EOR
CSB-EP483284EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Sigma factor-binding protein crl (crl) CSB-YP483285EOR
CSB-EP483285EOR
CSB-BP483285EOR
CSB-MP483285EOR
CSB-EP483285EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Lysophospholipid transporter lplT (lplT), partial CSB-YP483286EOR
CSB-EP483286EOR
CSB-BP483286EOR
CSB-MP483286EOR
CSB-EP483286EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Trp operon repressor (trpR) CSB-YP483287EOR
CSB-EP483287EOR
CSB-BP483287EOR
CSB-MP483287EOR
CSB-EP483287EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Outer membrane protein A (ompA), partial CSB-YP483288EOR
CSB-EP483288EOR
CSB-BP483288EOR
CSB-MP483288EOR
CSB-EP483288EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Curved DNA-binding protein (cbpA) CSB-YP483289EOR
CSB-EP483289EOR
CSB-BP483289EOR
CSB-MP483289EOR
CSB-EP483289EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP483290EOR
CSB-EP483290EOR
CSB-BP483290EOR
CSB-MP483290EOR
CSB-EP483290EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0059 membrane protein yebN (yebN), partial CSB-YP483291EOR1
CSB-EP483291EOR1
CSB-BP483291EOR1
CSB-MP483291EOR1
CSB-EP483291EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0301 protein yqgE (yqgE) CSB-YP483292EOR
CSB-EP483292EOR
CSB-BP483292EOR
CSB-MP483292EOR
CSB-EP483292EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Glyceraldehyde-3-phosphate dehydrogenase A (gapA) CSB-YP483293EOR
CSB-EP483293EOR
CSB-BP483293EOR
CSB-MP483293EOR
CSB-EP483293EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protein ves (ves) CSB-YP483294EOR
CSB-EP483294EOR
CSB-BP483294EOR
CSB-MP483294EOR
CSB-EP483294EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Integration host factor subunit alpha (ihfA) CSB-YP483295EOR
CSB-EP483295EOR
CSB-BP483295EOR
CSB-MP483295EOR
CSB-EP483295EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii HTH-type transcriptional repressor PurR (purR) CSB-YP483296EOR
CSB-EP483296EOR
CSB-BP483296EOR
CSB-MP483296EOR
CSB-EP483296EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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