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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa 30S ribosomal protein S15 (rpsO) CSB-YP464071XBN
CSB-EP464071XBN
CSB-BP464071XBN
CSB-MP464071XBN
CSB-EP464071XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 60 kDa chaperonin (groL), partial CSB-YP464072XBN
CSB-EP464072XBN
CSB-BP464072XBN
CSB-MP464072XBN
CSB-EP464072XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa C4-dicarboxylate transport protein (dctA), partial CSB-YP464073XBN
CSB-EP464073XBN
CSB-BP464073XBN
CSB-MP464073XBN
CSB-EP464073XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Gamma-glutamyl phosphate reductase (proA) CSB-YP464074XBN
CSB-EP464074XBN
CSB-BP464074XBN
CSB-MP464074XBN
CSB-EP464074XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Methionine--tRNA ligase (metG), partial CSB-YP464075XBN
CSB-EP464075XBN
CSB-BP464075XBN
CSB-MP464075XBN
CSB-EP464075XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Transcription elongation factor GreA (greA) CSB-YP464076XBN
CSB-EP464076XBN
CSB-BP464076XBN
CSB-MP464076XBN
CSB-EP464076XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa S-adenosylmethionine synthase (metK) CSB-YP464077XBN
CSB-EP464077XBN
CSB-BP464077XBN
CSB-MP464077XBN
CSB-EP464077XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L5 (rplE) CSB-YP464078XBN
CSB-EP464078XBN
CSB-BP464078XBN
CSB-MP464078XBN
CSB-EP464078XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S13 (rpsM) CSB-YP464079XBN
CSB-EP464079XBN
CSB-BP464079XBN
CSB-MP464079XBN
CSB-EP464079XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L28 (rpmB) CSB-YP464080XBN
CSB-EP464080XBN
CSB-BP464080XBN
CSB-MP464080XBN
CSB-EP464080XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP464081XBN
CSB-EP464081XBN
CSB-BP464081XBN
CSB-MP464081XBN
CSB-EP464081XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Enolase (eno) CSB-YP464082XBN
CSB-EP464082XBN
CSB-BP464082XBN
CSB-MP464082XBN
CSB-EP464082XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein translocase subunit SecA (secA), partial CSB-YP464083XBN
CSB-EP464083XBN
CSB-BP464083XBN
CSB-MP464083XBN
CSB-EP464083XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA mismatch repair protein mutL (mutL), partial CSB-YP464084XBN
CSB-EP464084XBN
CSB-BP464084XBN
CSB-MP464084XBN
CSB-EP464084XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Integration host factor subunit alpha (ihfA) CSB-YP464085XBN
CSB-EP464085XBN
CSB-BP464085XBN
CSB-MP464085XBN
CSB-EP464085XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA ligase (ligA), partial CSB-YP464086XBN
CSB-EP464086XBN
CSB-BP464086XBN
CSB-MP464086XBN
CSB-EP464086XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP464087XBN
CSB-EP464087XBN
CSB-BP464087XBN
CSB-MP464087XBN
CSB-EP464087XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Diaminopimelate epimerase (dapF) CSB-YP464088XBN
CSB-EP464088XBN
CSB-BP464088XBN
CSB-MP464088XBN
CSB-EP464088XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP464089XBN
CSB-EP464089XBN
CSB-BP464089XBN
CSB-MP464089XBN
CSB-EP464089XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glucans biosynthesis protein D (opgD), partial CSB-YP464090XBN
CSB-EP464090XBN
CSB-BP464090XBN
CSB-MP464090XBN
CSB-EP464090XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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