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  4. Recombinant Saccharomyces cerevisiae Glucan 1,3-beta-glucosidase (BGL2)

Recombinant Saccharomyces cerevisiae Glucan 1,3-beta-glucosidase (BGL2)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Glucan 1,3-beta-glucosidase (BGL2)
  • 品名簡(jiǎn)稱：
    Recombinant Saccharomyces cerevisiae BGL2 protein
  • 貨號(hào)：
    CSB-EP322229SVG
  • 說(shuō)明書：
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    BGL2
  • Uniprot No.：
  • 別名：
    Exo-1,3-beta-glucanase;GP29;Soluble cell wall protein 9
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 來(lái)源：
    E.coli
  • 分子量：
    38.6 kDa
  • 表達(dá)區(qū)域：
    24-313aa
  • 氨基酸序列
    IGELAFNLGVKNNDGTCKSTSDYETELQALKSYTSTVKVYAASDCNTLQNLGPAAEAEGFTIFVGVWPTDDSHYAAEKAALQTYLPKIKESTVAGFLVGSEALYRNDLTASQLSDKINDVRSVVADISDSDGKSYSGKQVGTVDSWNVLVAGYNSAVIEASDFVMANAFSYWQGQTMQNASYSFFDDIMQALQVIQSTKGSTDITFWVGETGWPTDGTNFESSYPSVDNAKQFWKEGICSMRAWGVNVIVFEAFDEDWKPNTSGTSDVEKHWGVFTSSDNLKYSLDCDFS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase.
  • 基因功能參考文獻(xiàn)：
    1. A screening aimed at identifying deletion mutants with reduced branching of beta-(1,3)-glucan through beta-(1,6)-glycosidic linkages among them revealed only two, the bgl2Delta and gas1Delta mutants, showing 15% and 70% reductions in the branching, respectively, compared to the wild-type strain. PMID: 28634239
    2. Functionally related Bgl2p and Gas1p are the major glucan transferases forming the molecular ensemble of yeast cell wall. PMID: 16986442
    3. role of high-molecular-weight polyphosphates in activation of glucan transferase Bg12p PMID: 18680912
    4. the first evidence for amyloid-like properties of yeast cell wall protein-glucantransferase Bgl2p. PMID: 19098439
  • 亞細(xì)胞定位：
    Secreted, cell wall. Note=Tightly bound to cell wall.
  • 蛋白家族：
    Glycosyl hydrolase 17 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YGR282C

    STRING: 4932.YGR282C



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