E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
NPY1; ENP; MAB4; At4g31820; F11C18.20; BTB/POZ domain-containing protein NPY1; Protein ENHANCER OF PINOID; Protein MACCHI-BOU 4; Protein NAKED PINS IN YUC MUTANTS 1
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產(chǎn)品評價
靶點詳情
功能:
May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Coregulates with PID the auxin-mediated plant organogenesis. Regulates cotyledon development through control of PIN1 polarity. May play an essential role in root gravitropic responses.
基因功能參考文獻:
Enhancer of Pinoid (ENP) protein specifically regulates cotyledon development through control of PIN1 polarity in concert with Pinoid. PMID: 16107478
a novel gene, MAB4, which is involved in organogenesis in concert with PID has been reported. It encodes a NPH3-like protein and is identical to ENP. [MACCHI-BOU 4] [MAB4] PMID: 17913786
NPY1 works with the protein kinase PID, which is homologous to PHOT1, to regulate auxin-mediated plant development. [NPY1] PMID: 18000043
亞細胞定位:
Late endosome. Note=Colocalized with PID.
蛋白家族:
NPH3 family
組織特異性:
Expressed mainly in the apical regions of embryos including cotyledon tips and the apical meristem. Highly expressed in primary root tips.