Flow cytometric analysis of polyamine modulated factor 1 expression in HepG2 cells using polyamine modulated factor 1 antibody. Green, isotype control; red, polyamine modulated factor 1.
Immunocytochemical staining of HepG2 cells with polyamine modulated factor 1 antibody. Nuclei were stained blue with DAPI; Polyamine modulated factor 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using polyamine modulated factor 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with polyamine modulated factor 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
亞細胞定位:
Nucleus. Chromosome, centromere, kinetochore. Note=Associated with the kinetochore.
組織特異性:
Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver.