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  4. CBS Recombinant Monoclonal Antibody

CBS Recombinant Monoclonal Antibody

  • 中文名稱：
    CBS Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA242578A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: MCF7 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), HEK293 whole cell lysate(30μg)
      All lanes:CBS antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 61 kDa
      Observed band size: 61 kDa
      Exposure time：2min
    • IHC image of CSB-RA242578A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA242578A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA242578A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    CBS
  • 別名：
    AI047524 antibody; AI303044 antibody; Beta thionase antibody; Beta-thionase antibody; Cbs antibody; Cbs cystathionine beta-synthase antibody; CBS_HUMAN antibody; Cystathionine beta synthase antibody; Cystathionine beta-synthase antibody; EC 4.2.1.22 antibody; HIP 4 antibody; HIP4 antibody; Methylcysteine synthase antibody; MGC18856 antibody; MGC18895 antibody; MGC37300 antibody; OTTHUMP00000109416 antibody; OTTHUMP00000109418 antibody; Serine sulfhydrase antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide from human CBS protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    14A11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
  • 相關(guān)疾?。?/div>
    Cystathionine beta-synthase deficiency (CBSD)
  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Cysteine synthase/cystathionine beta-synthase family
  • 組織特異性：
    In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1550

    OMIM: 236200

    KEGG: hsa:102724560

    UniGene: Hs.533013



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