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  4. DLST Antibody
  • 中文名稱(chēng)：
    DLST兔多克隆抗體
  • 貨號(hào)：
    CSB-PA861526LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Rat liver tissue, Rat kidney tissue
      All lanes: DLST antibody at 5.9μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 49, 40 kDa
      Observed band size: 49 kDa
    • IHC image of CSB-PA861526LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA861526LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA861526LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：
    Rabbit anti-Homo sapiens (Human) DLST Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    DLST
  • 別名：
    2-oxoglutarate dehydrogenase complex component E2 antibody; Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody; Dihydrolipoamide S succinyltransferase antibody; Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody; Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody; Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody; Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody; Dlst antibody; DLTS antibody; E2 antibody; E2K antibody; mitochondrial antibody; ODO2_HUMAN antibody; OGDC-E2 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human, Rat
  • 免疫原：
    Recombinant Human Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial protein (76-225AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，DLST Antibody (CSB-PA861526LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于DLST Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱(chēng) 應(yīng)用
    HRP CSB-PA861526LB01HU DLST Antibody, HRP conjugated ELISA
    FITC CSB-PA861526LC01HU DLST Antibody, FITC conjugated
    Biotin CSB-PA861526LD01HU DLST Antibody, Biotin conjugated ELISA
  • 克隆類(lèi)型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.
  • 基因功能參考文獻(xiàn)：
    1. RNAi knockdown of DLST led to decreased cell viability and induction of apoptosis in human T-ALL cell lines PMID: 26876595
    2. 2-oxoglutarate dehydrogenase complex (OGDHC), the key regulatory enzyme of Krebs cycle. [review] PMID: 22235656
    3. Data show that the dihydrolipoamide succinyltransferase gene is bifunctional, and truncated mRNA transcribed from it contributes to the biogenesis of the mitochondrial respiratory complexes. PMID: 12805207
    4. Association between late-onset Alzheimer disease shows strong linkage disequilibrium across the DLST locus. PMID: 15038610
  • 亞細(xì)胞定位：
    Mitochondrion matrix. Nucleus.
  • 蛋白家族：
    2-oxoacid dehydrogenase family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2911

    OMIM: 126063

    KEGG: hsa:1743

    STRING: 9606.ENSP00000335304

    UniGene: Hs.525459



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