Sheep arachidonic Acid(AA) ELISA kit

說(shuō)明書下載

產(chǎn)品詳情

產(chǎn)品描述：

綿羊花生四烯酸(AA)酶聯(lián)免疫試劑盒（CSB-EQ027590SH）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的AA含量。試劑盒檢測(cè)范圍為15.625 ng/mL-1000 ng/mL，靈敏度為13.797 ng/mL。該產(chǎn)品適用于科研領(lǐng)域中對(duì)綿羊模型炎癥機(jī)制、營(yíng)養(yǎng)代謝調(diào)控、藥物干預(yù)效果的評(píng)價(jià)，以及脂質(zhì)代謝相關(guān)通路的基礎(chǔ)研究，為體外實(shí)驗(yàn)提供可靠的定量數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

AA
種屬：

Ovis aries (Sheep)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Biochemicals
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	89-100
1:200	Average %	90
	Range %	85-95
1:400	Average %	100
	Range %	94-104
1:800	Average %	105
	Range %	97-110

回收率：

The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-106
EDTA plasma (n=4)	102	96-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進(jìn)展：

AA是一種抗氧化劑，最新研究發(fā)現(xiàn)，AA對(duì)于心血管疾病的預(yù)防有益。一項(xiàng)針對(duì)高危心血管患者的研究表明，AA的補(bǔ)充可以降低冠心病發(fā)生率和死亡率。