Sheep Interleukin 4(IL-4)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E15963Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL4含量。IL4是一種由活化的T淋巴細(xì)胞、肥大細(xì)胞、嗜堿性粒細(xì)胞產(chǎn)生的多效性細(xì)胞因子，在免疫調(diào)節(jié)中起關(guān)鍵作用。它通過誘導(dǎo)B細(xì)胞分化、促進(jìn)抗體產(chǎn)生和調(diào)節(jié)Th2細(xì)胞介導(dǎo)的免疫反應(yīng)，在過敏性疾病和腫瘤免疫中扮演重要角色。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于免疫學(xué)研究、炎癥性疾病模型分析、疫苗免疫效果評估等科研領(lǐng)域，可用于探究綿羊感染性疾病、寄生蟲免疫應(yīng)答及自身免疫機(jī)制，為其提供精準(zhǔn)的蛋白定量數(shù)據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL4 ELISA Kit; IL-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
縮寫：

IL4
Uniprot No.：

P30368
種屬：

Ovis aries (Sheep)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-95
1:2	Average %	102
	Range %	97-105
1:4	Average %	95
	Range %	90-97
1:8	Average %	97
	Range %	93-100

回收率：

The recovery of sheep IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	90-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
基因功能參考文獻(xiàn)：
1. Local production of the prototypic immunomodulatory cytokine IL-4 in transplanted corneal endothelium induces eosinophilia, inflammation, and rejection. PMID: 16081789
2. Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus infection. PMID: 27059919
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數(shù)據(jù)庫鏈接：

KEGG: oas:443327

UniGene: Oar.377