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  4. Sheep Interleukin 12,IL-12 ELISA Kit

Sheep Interleukin 12,IL-12 ELISA Kit

  • 中文名稱(chēng)：
    綿羊白介素12(IL-12)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14017Sh
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    綿羊白介素12(IL-12)酶聯(lián)免疫試劑盒（CSB-E14017Sh）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL12含量。IL12是重要的細(xì)胞因子，在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。它能促進(jìn)T細(xì)胞和NK細(xì)胞的活化和增殖，增強(qiáng)機(jī)體免疫應(yīng)答。研究聚焦其信號(hào)傳導(dǎo)機(jī)制、與疾病關(guān)聯(lián)。深入了解IL12機(jī)制，有望為免疫相關(guān)疾病如腫瘤、自身免疫病治療帶來(lái)新策略。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，本產(chǎn)品適用于綿羊免疫相關(guān)疾病的機(jī)制研究，如感染性疾病模型中細(xì)胞免疫應(yīng)答強(qiáng)度的動(dòng)態(tài)監(jiān)測(cè)、疫苗效力評(píng)估實(shí)驗(yàn)中的Th1型免疫激活分析，或炎癥性疾病研究中的細(xì)胞因子網(wǎng)絡(luò)調(diào)控解析，為獸醫(yī)科學(xué)及比較醫(yī)學(xué)研究提供精準(zhǔn)的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    IL-12
  • 種屬：
    Ovis aries (Sheep)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %87-94
    1:2Average %103
    Range %99-107
    1:4Average %93
    Range %87-99
    1:8Average %91
    Range %88-96
  • 回收率：
    The recovery of sheep IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-103
    EDTA plasma (n=4)9086-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.599 2.780 2.690 2.596
    10002.177 2.261 2.219 2.125
    5001.621 1.730 1.676 1.582
    2501.113 1.056 1.085 0.991
    1250.756 0.782 0.769 0.675
    62.50.399 0.411 0.405 0.311
    31.250.280 0.297 0.289 0.195
    00.097 0.090 0.094
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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