Rat tight junction protein 1 (ZO1) ELISA Kit

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文獻引用 (10) 說明書下載

中文名稱：

大鼠緊密連接蛋白1(ZO1)酶聯(lián)免疫試劑盒
貨號：

CSB-E17287r
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
規(guī)格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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引用文獻

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠緊密連接蛋白1(ZO1)酶聯(lián)免疫試劑盒（CSB-E17287r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的ZO1含量。大鼠緊密連接蛋白1（ZO-1）是緊密連接復(fù)合體的重要組成部分，參與維持上皮細胞間連接的穩(wěn)定性和腸道屏障功能。它通過連接膜蛋白與細胞骨架，調(diào)節(jié)細胞間通透性，對防止病原體和毒素進入體內(nèi)至關(guān)重要。研究顯示，ZO-1的表達與消化系統(tǒng)疾病的發(fā)生、發(fā)展及預(yù)后密切相關(guān)，其功能異?？赡軐?dǎo)致腸黏膜屏障破壞和多種消化系統(tǒng)疾病。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外研究ZO1在細胞模型或動物模型中的表達調(diào)控，例如藥物干預(yù)、氧化應(yīng)激或病原體感染等實驗條件下緊密連接功能的動態(tài)評估，為屏障相關(guān)疾病的分子機制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

DKFZp686M05161, MGC133289, ZO-1, tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein
縮寫：

ZO1
種屬：

Rattus norvegicus (Rat)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Tags & Cell Markers
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
Range %	86-95
1:2	Average %	102
Range %	97-107
1:4	Average %	91
Range %	85-97
1:8	Average %	97
Range %	91-103

回收率：

The recovery of rat ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.272	2.374	2.323	2.221
1000	1.846	1.967	1.907	1.805
500	1.496	1.392	1.444	1.342
250	0.962	0.946	0.954	0.852
125	0.578	0.596	0.587	0.485
62.5	0.364	0.351	0.358	0.256
31.2	0.225	0.230	0.228	0.126
0	0.101	0.102	0.102