Rat pigment epithelium-derived factor,PEDF ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (10) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠色素上皮衍生因子(PEDF)酶聯(lián)免疫試劑盒（CSB-E08819r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PEDF含量。PEDF（色素上皮衍生因子）是一種具有多種生物功能的分泌性糖蛋白，主要在視網(wǎng)膜色素上皮細(xì)胞中表達(dá)。它具有抗血管生成、抗炎和神經(jīng)營(yíng)養(yǎng)作用，對(duì)多種腫瘤細(xì)胞具有抑制作用。其研究機(jī)制涉及通過(guò)調(diào)節(jié)細(xì)胞增殖、凋亡和遷移等途徑抑制腫瘤生長(zhǎng)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于體外培養(yǎng)細(xì)胞分泌功能評(píng)估、疾病模型動(dòng)物血清標(biāo)志物分析以及組織微環(huán)境中PEDF動(dòng)態(tài)變化的科研探索，為血管生成調(diào)控、氧化應(yīng)激機(jī)制及神經(jīng)退行性疾病等基礎(chǔ)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

PEDF
Uniprot No.：

Q80ZA3
種屬：

Rattus norvegicus (Rat)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	87
	Range %	81-92
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	92
	Range %	85-97
1:8000	Average %	96
	Range %	91-102

回收率：

The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	87	83-91

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.