Rat enkephalin,ENK ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠腦啡肽(ENK)酶聯(lián)免疫試劑盒（CSB-E13325r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的ENK含量。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于神經(jīng)科學(xué)、藥理學(xué)等領(lǐng)域的基礎(chǔ)研究，例如探究疼痛機(jī)制模型、應(yīng)激反應(yīng)調(diào)控或藥物干預(yù)對(duì)ENK表達(dá)的影響，為神經(jīng)系統(tǒng)功能研究提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ENK
種屬：

Rattus norvegicus (Rat)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	103-111
1:2	Average %	95
	Range %	92-98
1:4	Average %	90
	Range %	86-95
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of rat ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	91	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.