Rat activated coagulation factor X(FXa) ELISA Kit

說(shuō)明書下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠活化凝血因子X(jué)(FXa)酶聯(lián)免疫試劑盒（CSB-EQ027251RA）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FXa含量。FXa即凝血因子X(jué)a，是凝血級(jí)聯(lián)反應(yīng)中的關(guān)鍵靶點(diǎn)。它在凝血過(guò)程中處于內(nèi)、外源凝血途徑交匯點(diǎn)，激活后促使凝血酶原轉(zhuǎn)化為凝血酶。對(duì)其研究機(jī)制主要聚焦于開發(fā)抑制劑，以抑制凝血過(guò)程，用于血栓性疾病防治。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，支持實(shí)驗(yàn)室開展凝血機(jī)制研究、抗凝藥物篩選、病理模型評(píng)估等方向?qū)嶒?yàn)，多樣本兼容性設(shè)計(jì)尤其適合系統(tǒng)性研究凝血因子在組織微環(huán)境中的動(dòng)態(tài)變化，為探索血栓性疾病、術(shù)后凝血異常等課題提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

FXa
種屬：

Rattus norvegicus (Rat)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	102
	Range %	98-109
1:400	Average %	90
	Range %	86-94
1:800	Average %	97
	Range %	92-101

回收率：

The recovery of rat FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.