Rat Platelet-derived growth factor DD(PDGF-DD) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠血小板衍生生長因子DD(PDGF-DD) 酶聯(lián)免疫試劑盒（CSB-EL017711RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-DD含量。PDGF - DD是一種血小板衍生生長因子異構(gòu)體。它在細胞的生長、增殖和遷移等過程中發(fā)揮重要作用。其研究機制主要聚焦于與受體結(jié)合后激活下游信號通路，影響細胞行為，在腫瘤、纖維化等疾病的發(fā)生發(fā)展中可能有潛在影響，是藥物研發(fā)的潛在靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域探究PDGF-DD在大鼠心血管疾病模型、器官纖維化機制、創(chuàng)傷修復(fù)實驗中的動態(tài)表達特征，通過血清或血漿樣本可系統(tǒng)性評估循環(huán)系統(tǒng)中PDGF-DD水平，而組織勻漿檢測則有助于解析特定器官或病變部位的局部蛋白表達模式，為相關(guān)分子機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-DD
種屬：

Rattus norvegicus (Rat)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-DD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-90
1:2	Average %	89
	Range %	85-96
1:4	Average %	90
	Range %	84-96
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of rat PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-103
EDTA plasma (n=4)	95	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.