Rat Copeptin ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠和肽素(copeptin)酶聯(lián)免疫試劑盒（CSB-E16335r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的copeptin含量。copeptin是精氨酸加壓素原的C末端片段，穩(wěn)定性好。其背景在于檢測它可間接反映精氨酸加壓素水平。研究機(jī)制方面，在一些疾病如心血管病、內(nèi)分泌疾病等中，copeptin水平會發(fā)生變化，可作為疾病診斷、病情評估和預(yù)后判斷的生物標(biāo)志物。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品適用于實(shí)驗(yàn)室研究中對大鼠模型在病理生理狀態(tài)下的和肽素水平動態(tài)監(jiān)測，例如心血管疾病機(jī)制探索、應(yīng)激反應(yīng)評估或腎臟功能相關(guān)研究，為深入解析相關(guān)疾病的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

copeptin
種屬：

Rattus norvegicus (Rat)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-102
1:2	Average %	94
	Range %	91-99
1:4	Average %	99
	Range %	96-106
1:8	Average %	97
	Range %	90-102

回收率：

The recovery of rat copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	88	82-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.