Rat Complement 4,C4 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠補(bǔ)體蛋白4(C4)酶聯(lián)免疫試劑盒（CSB-E08706r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的C4含量。試劑盒檢測(cè)范圍為12.5 ng/mL-800 ng/mL，適用于體外免疫學(xué)研究，如炎癥動(dòng)物模型中的血清C4動(dòng)態(tài)監(jiān)測(cè)、組織局部補(bǔ)體激活水平評(píng)估，或藥物干預(yù)下補(bǔ)體通路的功能研究，為免疫學(xué)、病理學(xué)及藥理學(xué)領(lǐng)域的科研工作提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

C4 ELISA Kit; C4aComplement C4 [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA Kit
縮寫：

C4
Uniprot No.：

P08649
種屬：

Rattus norvegicus (Rat)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	99
	Range %	96-102
1:400	Average %	87
	Range %	82-93
1:800	Average %	100
	Range %	96-104
1:1600	Average %	84
	Range %	81-89

回收率：

The recovery of rat C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	88	84-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory processes. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
基因功能參考文獻(xiàn)：
1. Our study provides the first evidence that sustained selective EPAC activation leads to an increase in TRPC3 and C4 protein expression and induces a proarrhythmic SOCE-like activity in adult rat ventricular cardiomyocytes PMID: 26219954
2. The expression of the complement component C3f and complement component 4A have potential for use in post-transplant diagnosis of rejection. PMID: 23613499
3. This study presents the genomic organization of the two C4 gene-containing modules and the expression of the two C4 genes in the rat (Rattus norvegicus). PMID: 15634920
亞細(xì)胞定位：

Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
數(shù)據(jù)庫鏈接：

KEGG: rno:24233

STRING: 10116.ENSRNOP00000037902

UniGene: Rn.155573