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  4. Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit

  • 中文名稱：
    大鼠Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08084r
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠Ⅰ型膠原(Col Ⅰ)酶聯(lián)免疫試劑盒（CSB-E08084r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Col Ⅰ含量。Col Ⅰ即Ⅰ型膠原蛋白，是細(xì)胞外基質(zhì)的主要成分。其背景在于它廣泛存在于多種組織，對(duì)維持組織正常結(jié)構(gòu)和功能起重要作用。研究機(jī)制方面，圍繞其在組織纖維化、腫瘤微環(huán)境等過(guò)程中的合成、降解及信號(hào)傳導(dǎo)途徑展開，旨在尋找相關(guān)疾病的治療靶點(diǎn)。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，適用于體外實(shí)驗(yàn)環(huán)境下對(duì)大鼠組織修復(fù)機(jī)制、纖維化疾病模型評(píng)估或骨骼代謝相關(guān)研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
  • 縮寫：
    Col Ⅰ
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 89  
    Range % 84-93  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of rat Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 88-98  
    EDTA plasma (n=4) 98 93-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.701 2.762 2.732 2.537  
    2500 1.903 1.954 1.929 1.734  
    1250 1.195 1.207 1.201 1.006  
    625 0.802 0.743 0.773 0.578  
    312 0.489 0.466 0.478 0.283  
    156 0.376 0.359 0.368 0.173  
    78 0.258 0.243 0.251 0.056  
    0 0.201 0.189 0.195 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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