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  4. Rat C-Reactive Protein,CRP ELISA Kit

Rat C-Reactive Protein,CRP ELISA Kit

  • 中文名稱(chēng)：
    大鼠C反應(yīng)蛋白(CRP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07922r
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissuehomogenates and cell lysates
  • 檢測(cè)范圍：
    2.74 ng/ml-2000 ng/ml
  • 靈敏度：
    2.74ng/ml
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠C反應(yīng)蛋白(CRP)酶聯(lián)免疫試劑盒（CSB-E07922r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的CRP含量。CRP即C反應(yīng)蛋白，是一種急性時(shí)相反應(yīng)蛋白。在炎癥、感染、創(chuàng)傷等刺激下，肝臟合成CRP增加。其研究機(jī)制主要聚焦于它與炎癥反應(yīng)、心血管疾病等密切關(guān)聯(lián)，可作為疾病診斷、病情監(jiān)測(cè)和預(yù)后評(píng)估的重要標(biāo)志物。試劑盒檢測(cè)范圍為2.74 ng/ml-2000 ng/ml，該產(chǎn)品可廣泛應(yīng)用于科研領(lǐng)域，如炎癥性疾病機(jī)制研究、實(shí)驗(yàn)動(dòng)物模型炎癥進(jìn)程監(jiān)測(cè)、藥物或化合物抗炎效果評(píng)估等方向，為體外實(shí)驗(yàn)中CRP動(dòng)態(tài)變化提供標(biāo)準(zhǔn)化檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Crp ELISA kit; Ptx1C-reactive protein ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    85

    Range %

    81-92

    1:200

    Average %

    95

    Range %

    88-102

    1:400

    Average %

    101

    Range %

    96-107

    1:800

    Average %

    98

    Range %

    91-104

  • 回收率：

    The recovery of rat CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    89-100

    EDTA plasma (n=4)

    96

    90-102

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    2000

    3.427

    3.309

    3.368

    3.287

    666.66

    2.889

    2.962

    2.925

    2.844

    222.22

    2.274

    2.388

    2.331

    2.250

    74.07

    1.409

    1.485

    1.447

    1.366

    24.7

    0.862

    0.832

    0.847

    0.766

    8.23

    0.281

    0.296

    0.288

    0.207

    2.74

    0.150

    0.147

    0.148

    0.068

    0

    0.079

    0.083

    0.081

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    CRP是C反應(yīng)蛋白的簡(jiǎn)稱(chēng)，是一種急性反應(yīng)蛋白。最新研究表明，CRP不僅可以作為心血管疾病等疾病的生物標(biāo)志物，還與COVID-19感染的嚴(yán)重程度相關(guān)。此外，研究還發(fā)現(xiàn)CRP參與多種疾病的發(fā)生發(fā)展，包括癌癥、自身免疫疾病和神經(jīng)退行性疾病等。
  • 功能：
    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • 基因功能參考文獻(xiàn)：
    1. coordinated upregulation of genes encoding CRP, interleukin-6 (IL-6), HNF1alpha, and HNF4alpha in the livers and white adipose tissue (WAT) of chronic renal failure rats, as compared to the pair-fed and control animals. PMID: 29330688
    2. CRP: varied between DJOS and SHAM groups maintained on HF/HF, CD/CD, and CD/HF. PMID: 29849871
    3. Fibrinopeptide A induces CRP expression in Vascular Smooth Muscle Cells via Reactive Oxygen Species-ERK1/2/p38-NF-kappaB signal pathway. PMID: 29768263
    4. Knockdown of ZFAS1 or miR-150 overexpression effectively relieved AMI-induced myocardial infarction in AMI-1 week rats. The ZFAS1/miR-150 axis was involved in the molecular mechanism of AMI induced cardiomyocytes apoptosis via regulating CRP PMID: 28295592
    5. Epigallocatechin-3-gallate inhibits Ang II-induced CRP generation by interfering with AT1-ROS-ERK1/2 signal pathway in hepatocytes, preventing inflammation. PMID: 27492718
    6. Salvianolic acid B exerted anti-steatotic and anti-inflammatory effects against alcoholic liver injury by inducing SIRT1-mediated inhibition of CRP and ChREBP expression. PMID: 27989594
    7. Rosiglitazone inhibits angiotensin II-induced C-reactive protein generation by interfering with AT1-ROS-ERK1/2/JNK signal pathway and up-regulating PPARgamma expression in hepatocytes PMID: 26741265
    8. These findings provide evidence that, in the presence of high CRP levels, rosuvastatin exhibits significant anti-inflammatory, anti-oxidative, and renoprotective effects. PMID: 25536316
    9. Data show that palmitic acid significantly stimulated mRNA and protein expression of C-reactive protein, inducible nitric oxide synthase and tumor necrosis factor-alpha in vascular smooth muscle cells in time- and concentration-dependent manners. PMID: 25242580
    10. No correlation was found between CRP levels and microvascular or macrovascular dysfunction in adjuvant-induced arthritis. PMID: 25708190
    11. Homocysteine initiates an inflammatory response in VSMCs by stimulating CRP production via the NMDAr-ROS-ERK1/2/p38-NF-kappaB signal pathway. PMID: 25016361
    12. CRP and IgM depositions demonstrate a parallel time course throughout the reperfusion to hepatocellular damage, inflammatory response and activated complement deposition in an ischemia-reperfusion model. PMID: 24642533
    13. These demonstrate that aldosterone is able to induce CRP expression via MR-ROS-ERK1/2-NF-kappaB signal pathway in VSMCs, which provides a new evidence for the proinflammatory and proatherosclerotic effects of aldosterone. PMID: 25109280
    14. Monomeric CRP localized with inflammatory cells in infarcted myocardium. Intravenous pCRP administration significantly enhanced leukocyte rolling, adhesion, and transmigration via localized dissociation to mCRP in inflamed cremaster muscle. PMID: 24982116
    15. In older animals, there is depressed PAP expression related to a blunted inflammatory response in acute pancreatitis which is associated with worsened bacterial infiltration and higher C-reactive protein level PMID: 22807607
    16. PDIA3 might be potentially involve into the occurence and development of acute rejection response in renal transplantation and increased plasma IL-2, IL-4, IL-6, and CRP levels play an important role to prevent acute kidney allograft rejection in rats. PMID: 22201020
    17. C-reactive protein down-regulates endothelial nitric oxide synthase expression and promotes apoptosis in endothelial progenitor cells through receptor for advanced glycation end-products PMID: 22301267
    18. After attachment of polymeric CRP to the surface of necrotic cardiomyocytes, generation of monomeric CRP was demonstrated over a period of 18 h. PMID: 21856781
    19. This finding could indicate a possible role for CRP in the progression of small vessel disease in the brain and provide a therapeutic target PMID: 21840509
    20. C-reactive protein and tumour necrosis factor-alpha are elevated in collagen-induced arthritis in Dark Agouti rats PMID: 21633874
    21. Increased levels of CRP are an early sign of alcoholic fatty liver in rats. PMID: 21806828
    22. C-reactive protein exerts a proinflammatory action via Toll-like receptor 4-dependent signaling pathway (AT(1)R-p38 MAPK-TLR4-PKCalpha) in rat vascular smooth muscle cells. PMID: 20332628
    23. CRP may cause insulin resistance by increasing IRS-1 phosphorylation at Ser(307) and Ser(612) via JNK and ERK1/2, respectively, leading to impaired insulin-stimulated glucose uptake, GLUT4 translocation, and glycogen synthesis PMID: 17279354
    24. the Ala12 allele of PPARG2 may be an independent factor associated with low serum hs-CRP levels in Japanese females PMID: 17574226
    25. levels of C-reactive protein and adiponectin are improved after exercise training in diabetic rats PMID: 17660720
    26. CRP and IgM depositions correlated well with local complement activation, which suggests a role of these molecules in complement activation PMID: 17981193
    27. CRP levels and cardiovascular disease risk and the long term clinical outcome after acute coronary syndrome but such correlation has yet to be proven regarding CRP and atherosclerosis. PMID: 18254451
    28. C-reactive protein stimulates superoxide anion release and tissue factor activity in vivo PMID: 18621373
    29. CRP at clinically relevant concentrations (10 to 20 microg/mL) causes a disruption of the blood-brain barrier PMID: 19246692
    30. The results suggest that CRP contributes to memory loss and early phase of pathogenesis of Alzheimer disease PMID: 19263040
    31. The results demonstrate that ET-1 induces CPR production in VSMCs via ET(A) receptor followed by ROS and MAPK signal pathway. PMID: 19748488

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Pentraxin family
  • 組織特異性：
    Found in plasma.
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