Rat Anti-Cyclic Citrullinated Peptide Antibody(Anti-CCP-antibody)ELISA Kit

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠抗環(huán)胍氨酸肽抗體(Anti-CCP-antibody)酶聯(lián)免疫試劑盒（CSB-E13830r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CCP Ab含量。CCP Ab即抗環(huán)瓜氨酸肽抗體，在類風濕關節(jié)炎研究中是重要靶點。它對類風濕關節(jié)炎診斷特異性高，能較早出現(xiàn)于疾病進程中。其研究機制主要是通過識別瓜氨酸化抗原，觸發(fā)免疫反應，參與關節(jié)炎癥破壞，助力疾病早期診斷與病情監(jiān)測。試劑盒檢測范圍為3.12 IU/mL--200 IU/mL，為類風濕性關節(jié)炎等自身免疫性疾病機制研究提供高效工具；檢測 Anti - CCP 抗體對疾病早期診斷及病程監(jiān)測具有重要科研價值；適用于體外實驗中定量評估血清、血漿等生物樣本中抗體水平；科研人員可通過該產品開展疾病模型中的抗體動態(tài)變化研究、藥物干預效果評價或探索 CCP 抗體與關節(jié)炎癥的相關性，為免疫病理機制解析及新型治療策略開發(fā)提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CCP Ab
種屬：

Rattus norvegicus (Rat)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	85-105
1:2	Average %	97
	Range %	84-105
1:4	Average %	96
	Range %	92-100
1:8	Average %	95
	Range %	88-105

回收率：

The recovery of rat anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-96
EDTA plasma (n=4)	94	85-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.