Rabbit cardiac troponin Ⅰ,cTn-ⅠELISA Kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯(lián)免疫試劑盒（CSB-E11286Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNNI3含量。TNNI3是重要的心肌肌鈣蛋白亞基，在心肌收縮和舒張調(diào)節(jié)中發(fā)揮關(guān)鍵作用。其異常與多種心臟疾病密切相關(guān)，如心肌梗死、心肌病等。科研人員通過(guò)研究其表達(dá)、結(jié)構(gòu)及功能機(jī)制，探索基于TNNI3的診斷標(biāo)志物和治療靶點(diǎn)。試劑盒檢測(cè)范圍為47 pg/mL-3000 pg/mL，該產(chǎn)品適用于基礎(chǔ)科研中與心肌損傷相關(guān)的動(dòng)物模型研究，如心肌缺血再灌注損傷、藥物心臟毒性評(píng)估或心血管疾病機(jī)制探討等領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
縮寫(xiě)：

TNNI3
Uniprot No.：

P02646
種屬：

Oryctolagus cuniculus (Rabbit)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	96
	Range %	92-99
1:4	Average %	94
	Range %	89-98
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rabbit cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-94
EDTA plasma (n=4)	89	85-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
蛋白家族：

Troponin I family