Rabbit Thyroid Stimulating Hormone,TSH ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子促甲狀腺素(TSH)酶聯(lián)免疫試劑盒（CSB-E06916Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TSH含量。促甲狀腺激素（TSH）是腺垂體分泌的促進甲狀腺生長和機能的激素。其分泌受下丘腦分泌的促甲狀腺激素釋放激素調(diào)節(jié)。研究機制方面，TSH 與甲狀腺濾泡上皮細胞膜上受體結(jié)合，刺激甲狀腺激素合成與釋放，對維持甲狀腺的正常功能至關重要。試劑盒檢測范圍為0.5 μIU/mL-20 μIU/mL，適用于科研場景中甲狀腺相關疾病的動物模型研究、藥物干預對激素分泌的影響分析或基礎內(nèi)分泌機制探索；為實驗動物TSH水平監(jiān)測提供可靠工具；特別推薦用于小動物內(nèi)分泌系統(tǒng)研究的縱向觀察或跨組學關聯(lián)分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TSH
種屬：

Oryctolagus cuniculus (Rabbit)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	84
	Range %	80-88
1:4	Average %	94
	Range %	90-98
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of rabbit TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	96	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.