日本高潮视频在线观看-亚洲中文字幕永不卡-精品亚洲一区二区三区-伦理片夜夜躁狠狠躁日日躁-日本最新免费不卡二区-国产精品口爆一区二区三区-av一区二区三区高清-大鸡巴疯狂抽插小穴视频-日韩中文国产在线观看免费视频

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rabbit Leptin,LEP ELISA Kit

Rabbit Leptin,LEP ELISA Kit

  • 中文名稱：
    兔子瘦素(LEP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06971Rb
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子瘦素(LEP)酶聯(lián)免疫試劑盒（CSB-E06971Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LEP含量。LEP即瘦素，其編碼基因是肥胖基因。它由脂肪組織分泌，可作用于下丘腦調節(jié)能量代謝和食欲。研究機制上，通過與瘦素受體結合激活信號通路，調控神經(jīng)肽表達以影響進食行為等，在體重和代謝平衡調節(jié)中至關重要。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品適用于科研領域中對兔模型能量代謝機制、脂肪組織功能、肥胖相關疾病機制的研究，尤其適用于評估脂肪細胞分泌活性或藥物干預后LEP的動態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 91-99  
    1:200 Average % 93  
    Range % 88-97  
    1:400 Average % 85  
    Range % 80-89  
    1:800 Average % 90  
    Range % 84-96  
  • 回收率：
    The recovery of rabbit LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-105  
    EDTA plasma (n=4) 87 83-97  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.097 0.098 0.098    
    500 0.267 0.271 0.269    
    250 0.477 0.466 0.472    
    125 0.703 0.691 0.697    
    62.5 1.104 1.091 1.098    
    31.25 1.412 1.429 1.421    
    15.6 1.606 1.705 1.656    
    0 1.813 1.915 1.864    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價



×
生命科學計算平臺