Rabbit Interleukin 17,IL-17 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔白介素17(IL-17)酶聯(lián)免疫試劑盒（CSB-E08058Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL17含量。IL-17 是重要的細(xì)胞因子，在免疫應(yīng)答和炎癥反應(yīng)中起關(guān)鍵作用。它與自身免疫性疾病、感染性疾病等多種疾病相關(guān)。研究機(jī)制方面，主要聚焦其信號通路、與其他細(xì)胞因子相互作用等，以探索通過干預(yù) IL-17 治療相關(guān)疾病的方法。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于炎癥模型構(gòu)建、免疫應(yīng)答機(jī)制研究、藥物干預(yù)效果評價等科研場景，例如通過分析感染或自身免疫疾病模型中血清IL - 17水平變化，或探究特定組織內(nèi)細(xì)胞因子表達(dá)特征，為免疫相關(guān)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-17
Uniprot No.：

G1SLF2
種屬：

Oryctolagus cuniculus (Rabbit)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	81-96
1:2	Average %	94
	Range %	85-105
1:4	Average %	95
	Range %	87-102
1:8	Average %	91
	Range %	83-99

回收率：

The recovery of rabbit IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-100
EDTA plasma (n=4)	95	84-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.