Rabbit Immunoglobulin M,IgM ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

兔子免疫球蛋白M(IgM)酶聯(lián)免疫試劑盒（CSB-E06950Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgM含量。IgM是人體免疫系統(tǒng)中重要的免疫球蛋白。在它是個體發(fā)育過程中最早合成和分泌的抗體。其研究機(jī)制主要聚焦在免疫識別與防御，可快速結(jié)合病原體激活補(bǔ)體系統(tǒng)，在抗感染初期發(fā)揮關(guān)鍵作用，也在自身免疫病等方面有重要研究價(jià)值。試劑盒檢測范圍為15.6 ng/mL-4000 ng/mL，在評估實(shí)驗(yàn)動物免疫狀態(tài)或感染模型建立等科研場景中廣泛應(yīng)用；適用于疫苗開發(fā)、感染性疾病動物模型研究、免疫抑制劑效果評估等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgM
種屬：

Oryctolagus cuniculus (Rabbit)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	87-105
1:2000	Average %	92
	Range %	85-100
1:4000	Average %	93
	Range %	89-100
1:8000	Average %	94
	Range %	85-99

回收率：

The recovery of rabbit IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.