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Rabbit Bone Alkaline Phosphatase (BALP) ELISA Kit

  • 中文名稱:
    兔子骨堿性磷酸酶(BALP)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E15042Rb
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.156 mIU/mL-10 mIU/mL
  • 靈敏度:
    0.039 mIU/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔子骨堿性磷酸酶(BALP)酶聯(lián)免疫試劑盒(CSB-E15042Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的BALP含量。BALP即骨堿性磷酸酶,它是成骨細胞的表型標志物。在骨代謝中,成骨細胞分泌BALP參與骨基質(zhì)的合成與礦化。研究通過檢測其水平來反映成骨細胞活性及骨形成狀況,在骨疾病如骨質(zhì)疏松、佝僂病等的診斷與病情監(jiān)測中有重要意義。試劑盒檢測范圍為0.156 mIU/mL-10 mIU/mL,本試劑盒適用于骨代謝相關動物模型研究,如骨質(zhì)疏松癥機制探索、骨折愈合進程監(jiān)測、骨再生藥物療效評估等科研場景,為體外定量分析提供穩(wěn)定可靠的數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    BALP
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 反應時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %88-101
    1:2Average %88
    Range %81-92
    1:4Average %89
    Range %85-94
    1:8Average %98
    Range %90-101
  • 回收率:
    The recovery of rabbit BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10395-108
    EDTA plasma (n=4)9991-103
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    102.136 2.087 2.112 2.009
    51.839 1.783 1.811 1.708
    2.51.513 1.488 1.501 1.398
    1.251.079 1.028 1.054 0.951
    0.6250.752 0.722 0.737 0.634
    0.3120.562 0.554 0.558 0.455
    0.1560.335 0.327 0.331 0.228
    00.102 0.104 0.103
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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