Pig interleukin 2,IL-2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E06783p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的免疫細(xì)胞因子，在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。其背景在于它能促進(jìn)T細(xì)胞、NK細(xì)胞等免疫細(xì)胞增殖與活化。研究機(jī)制上，IL2通過與受體結(jié)合激活下游信號通路，增強(qiáng)機(jī)體免疫功能，在腫瘤免疫治療等領(lǐng)域研究廣泛。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領(lǐng)域中對豬類免疫系統(tǒng)功能的評估，例如疫苗研發(fā)中免疫激活效果分析、傳染病模型中細(xì)胞免疫反應(yīng)監(jiān)測，或通過檢測組織內(nèi)IL - 2表達(dá)水平探究炎癥性疾病的分子機(jī)制。為畜牧獸醫(yī)研究、比較免疫學(xué)及動(dòng)物疾病模型構(gòu)建提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
縮寫：

IL2
Uniprot No.：

P26891
種屬：

Sus scrofa (Pig)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	104
	Range %	97-111
1:2	Average %	90
	Range %	85-96
1:4	Average %	95
	Range %	88-101
1:8	Average %	89
	Range %	82-96

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.687	2.634	2.660	2.531
500	1.707	1.667	1.687	1.558
250	0.996	1.028	1.012	0.883
125	0.664	0.629	0.647	0.518
62.5	0.431	0.421	0.426	0.297
31.2	0.241	0.253	0.247	0.118
15.6	0.198	0.184	0.191	0.062
0	0.133	0.125	0.129

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能參考文獻(xiàn)：
1. We observed that besides TCR stimulation Tregs require IL-2 for activation. PMID: 21530576
2. a parameter of early mRNA expression during intestinal ischemia reperfusion injury PMID: 15050130
3. The isolation and characterization of interleukin 2 from Tibetan swine are reported. PMID: 16195934
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-2 family
數(shù)據(jù)庫鏈接：

KEGG: ssc:396868

STRING: 9823.ENSSSCP00000009697

UniGene: Ssc.16224