Pig collagen type I (COLI) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬I型膠原(COLI)酶聯(lián)免疫試劑盒（CSB-EQ027853PI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的COLI含量。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，適用于動(dòng)物模型研究、組織工程開發(fā)、骨骼發(fā)育機(jī)制探索以及膠原代謝相關(guān)疾病的病理機(jī)制分析等科研場(chǎng)景，為研究豬源性生物材料或疾病模型提供可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

collagen type 1 (COL1),_x000D_ collagen type I (COLI)
縮寫：

COLI
種屬：

Sus scrofa (Pig)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig COLⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	86-91
1:2	Average %	89
	Range %	81-95
1:4	Average %	98
	Range %	93-104
1:8	Average %	102
	Range %	98-106

回收率：

The recovery of pig COLⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-109
EDTA plasma (n=4)	92	88-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.