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Your Good Partner in Biology Research

Pig calprotectin (CALP) ELISA kit

  • 中文名稱:
    豬鈣衛(wèi)蛋白(CALP)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EQ013485PI
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬鈣衛(wèi)蛋白(CALP)酶聯(lián)免疫試劑盒(CSB-EQ013485PI)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CALP含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,適用于動物疾病模型研究(如腸道炎癥、感染性疾病)、免疫機制探索或藥物開發(fā)中炎癥標(biāo)志物的動態(tài)監(jiān)測。適用于獸醫(yī)病理學(xué)、比較醫(yī)學(xué)及畜牧養(yǎng)殖領(lǐng)域的科研分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    CALP
  • 種屬:
    Sus scrofa (Pig)
  • 反應(yīng)時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:100 Average % 96    
    Range % 91-102    
    1:200 Average % 85    
    Range % 81-89    
    1:400 Average % 103    
    Range % 98-107    
    1:800 Average % 87    
    Range % 83-92    
  • 回收率:
    The recovery of pig CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 94 88-97    
    EDTA plasma (n=4) 106 102-108    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    20 0.099 0.101 0.100      
    10 0.160 0.168 0.164      
    5 0.241 0.255 0.248      
    2.5 0.365 0.373 0.369      
    1.25 0.544 0.556 0.550      
    0.625 0.713 0.755 0.734      
    0.312 0.984 1.044 1.014      
    0 1.765 1.834 1.800      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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