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Pig anti-Mullerian hormone (AMH) ELISA kit

  • 中文名稱:
    豬繆勒管抑制物質(zhì)/抗繆勒管激素(AMH)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13408p
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.25 ng/mL-50 ng/mL
  • 靈敏度:
    1.25 ng/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬繆勒管抑制物質(zhì)/抗繆勒管激素(AMH)酶聯(lián)免疫試劑盒(CSB-E13408p)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的AMH含量??箍娎展芗に兀ˋMH)是轉(zhuǎn)化生長因子β超家族成員。在女性卵泡生長發(fā)育中起重要調(diào)控作用,可反映卵巢儲備功能。研究機(jī)制上,其參與調(diào)節(jié)卵泡募集和生長,且水平與竇前卵泡和小竇卵泡數(shù)量相關(guān),是評估卵巢功能的關(guān)鍵指標(biāo)。試劑盒檢測范圍為1.25 ng/mL-50 ng/mL,適用于動物生殖生理研究、繁殖效率評估、卵巢功能分析等科研領(lǐng)域,特別為豬科動物生殖內(nèi)分泌研究提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 反應(yīng)時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 89-102  
    1:2 Average % 93  
    Range % 90-103  
    1:4 Average % 90  
    Range % 85-100  
    1:8 Average % 93  
    Range % 83-107  
  • 回收率:
    The recovery of pig AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-103  
    EDTA plasma (n=4) 97 88-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.134 0.139 0.137    
    25 0.227 0.238 0.233    
    11.5 0.330 0.343 0.337    
    3.75 0.611 0.592 0.602    
    1.25 1.264 1.214 1.239    
    0 2.686 2.635 2.661    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

平均分:
4.0分 - 1 個評價

樣品類型:血清

樣品信息:其他 豬

稀釋比:沒有稀釋

產(chǎn)品評價: CSB-E13408p試劑盒用于檢測豬血清樣本,標(biāo)準(zhǔn)品無需倍比稀釋,直接使用即可,大大簡化了實驗流程。孵育時間比常規(guī)試劑盒更短,整個檢測過程高效省時。標(biāo)準(zhǔn)曲線OD值梯度良好(1.457-0.091),四參數(shù)擬合R2=0.99,曲線質(zhì)量優(yōu)秀。盡管實測濃度略低于預(yù)期,但廠家解釋為正?,F(xiàn)象,數(shù)據(jù)仍然可信。推薦需要快速、穩(wěn)定檢測的實驗室使用。

By 潘老師

靶點詳情

  • 功能:
    This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    TGF-beta family
  • 數(shù)據(jù)庫鏈接:

    UniGene: Ssc.97577



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