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  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Pig Glucagon(GC) ELISA Kit
  • 中文名稱：
    豬胰高血糖素(GC)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04854p
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    125 pg/mL-2000 pg/mL
  • 靈敏度：
    62.5 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬胰高血糖素(GC)酶聯(lián)免疫試劑盒（CSB-E04854p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GCG含量。GCG 即胰高血糖素基因，其編碼的胰高血糖素在血糖調(diào)節(jié)中作用關(guān)鍵，與胰島素共同維持血糖穩(wěn)態(tài)。研究機(jī)制方面，聚焦于其信號(hào)通路，通過與受體結(jié)合發(fā)揮功能，還用于研發(fā)調(diào)控血糖的藥物，在糖尿病等代謝疾病治療上有重要研究?jī)r(jià)值。試劑盒檢測(cè)范圍為125 pg/mL-2000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于動(dòng)物營(yíng)養(yǎng)學(xué)、代謝性疾病模型研究、激素調(diào)控機(jī)制探索等科研領(lǐng)域，例如通過檢測(cè)不同飼喂條件或疾病狀態(tài)下豬的GC動(dòng)態(tài)變化，為糖代謝研究、藥效評(píng)價(jià)及生理調(diào)控提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GCGGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA Kit; GRPP); Oxyntomodulin ELISA Kit; OXM ELISA Kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA Kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA Kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA Kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA Kit; GLP-2)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %85-96
    1:2Average %95
    Range %92-102
    1:4Average %84
    Range %80-88
    1:8Average %90
    Range %85-96
  • 回收率：
    The recovery of pig GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-97
    EDTA plasma (n=4)9690-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    20000.120 0.111 0.116
    10000.315 0.340 0.328
    5000.601 0.611 0.606
    2501.018 0.945 0.982
    1252.036 1.923 1.980
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：其他 豬

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)： 我用CSB E04854p檢測(cè)血清中g(shù)c（豬），標(biāo)準(zhǔn)曲線OD值3.10，2.87，1.67，0.82，0.46，0.25，樣本OD只大概為1.0，在標(biāo)曲范圍內(nèi)，整體平行也不錯(cuò)。之前有對(duì)比科鹿的，科鹿的結(jié)果完全沒辦法看，組內(nèi)差異非常的大，最終還是買了華美的。

By 高老師

靶點(diǎn)詳情

  • 功能：
    Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.; Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis.; Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.; Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.; May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that casein, safflower oil, sucrose, and sweetening agent rebaudioside A stimulate GLP1 release/secretion from enteroendocrine cells. (GLP1 = glucagon-like peptide 1, a peptide fragment derived from proglucagon) PMID: 27142747
    2. The patterns of colocalization of the K cell marker, glucagon-like insulinotropic peptide, and L cell markers, glucagon like peptide-1 and peptide YY, in enteroendocrine cells of the small intestine and colon of mouse and pig, were investigated. PMID: 25378285
  • 亞細(xì)胞定位：
    Secreted.; [Glucagon-like peptide 1]: Secreted.
  • 蛋白家族：
    Glucagon family
  • 組織特異性：
    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
  • 數(shù)據(jù)庫(kù)鏈接：


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