Pig Epithelial growth factor,EGF ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒（CSB-E06788p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的EGF含量。EGF（表皮生長(zhǎng)因子）是EGF家族的創(chuàng)始成員，具有與家族成員高度相似的結(jié)構(gòu)和功能。EGF是一種低分子量多肽，最初從小鼠頜下腺中純化，后在多種人體組織中發(fā)現(xiàn)。EGF通過(guò)與細(xì)胞表面的EGFR受體結(jié)合，刺激細(xì)胞增殖、分化和存活，在傷口愈合、組織修復(fù)等方面發(fā)揮重要作用。試劑盒檢測(cè)范圍為250 pg/mL-10000 pg/mL，可廣泛應(yīng)用于動(dòng)物營(yíng)養(yǎng)學(xué)、生殖生理學(xué)、免疫學(xué)等基礎(chǔ)研究領(lǐng)域，例如探究飼料添加劑對(duì)腸道黏膜修復(fù)的影響、評(píng)估應(yīng)激狀態(tài)下EGF的動(dòng)態(tài)變化，或解析特定疾病模型中生長(zhǎng)因子調(diào)控機(jī)制本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

EGF
Uniprot No.：

F1S137
種屬：

Sus scrofa (Pig)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	93
	Range %	90-96
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	83-90

回收率：

The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	101	99-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.