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Mouse thyroxine,T4 ELISA Kit

  • 中文名稱:
    小鼠甲狀腺素(T4)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E05083m
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    20 ng/mL-320 ng/mL
  • 靈敏度:
    20 ng/mL
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠甲狀腺素(T4)酶聯(lián)免疫試劑盒(CSB-E05083m)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的T4含量。小鼠甲狀腺素T4是甲狀腺分泌的關(guān)鍵激素,主要參與調(diào)節(jié)新陳代謝、生長發(fā)育和神經(jīng)系統(tǒng)功能。其作用機(jī)制涉及直接作用于破骨細(xì)胞和成骨細(xì)胞,影響骨鈣代謝,并通過TSH受體調(diào)節(jié)骨形成和吸收。此外,T4還與季節(jié)性繁殖、腸道菌群和心臟功能等方面有關(guān)。試劑盒檢測范圍為20 ng/mL-320 ng/mL,靈敏度為20 ng/mL。常用于科研領(lǐng)域的小鼠模型甲狀腺相關(guān)疾病研究、藥物作用評估及代謝調(diào)控機(jī)制探索;適用于實驗室對內(nèi)分泌系統(tǒng)功能研究、藥物干預(yù)效果評價以及代謝性疾?。ㄈ绶逝?、糖尿?。┠P偷臋C(jī)制分析等場景本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    T4
  • 種屬:
    Mus musculus (Mouse)
  • 反應(yīng)時間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:

     

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 標(biāo)準(zhǔn)曲線:

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本試劑盒所含材料:
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-mouse T4 antibody.
      • Standard 6 x 1 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-conjugated T4 1 x 6 ml
      • HRP-avidin 1 x 6 ml -- HRP catalyzes the TMB to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:沒有稀釋

產(chǎn)品評價: 我用CSB-E05083檢測正常小鼠的血清樣本,標(biāo)準(zhǔn)品的OD值范圍為2.408~0.162,標(biāo)準(zhǔn)曲線的R值>0.99,樣本的OD值范圍在0.4~0.7。試劑盒操作步驟清晰明了,適合科研

By 李老師

相關(guān)問答

 常見問題解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

靶點詳情

  • 最新研究進(jìn)展:
    近期研究發(fā)現(xiàn),T4蛋白在維持人類健康和免疫系統(tǒng)中發(fā)揮著重要的作用,特別是對于甲狀腺激素代謝的平衡具有重要作用。


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