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Mouse procollagen type III (HPCⅢ) ELISA Kit

  • 中文名稱:
    小鼠Ⅲ型前膠原(HPCⅢ)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E17125m
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠Ⅲ型前膠原(HPCⅢ)酶聯(lián)免疫試劑盒(CSB-E17125m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的HPCⅢ含量。小鼠Ⅲ型前膠原(HPCⅢ)是細(xì)胞外基質(zhì)(ECM)的重要組成部分,其合成與降解失衡是肝纖維化的關(guān)鍵因素。砷暴露可誘導(dǎo)HPCⅢ表達(dá)增加,促進(jìn)肝星狀細(xì)胞(HSC)活化,導(dǎo)致ECM過度沉積,進(jìn)而引發(fā)肝纖維化。研究揭示,HPCⅢ在肝纖維化發(fā)生發(fā)展中扮演著重要角色。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,適用于基礎(chǔ)科研中纖維化疾病機(jī)制或組織修復(fù)相關(guān)研究。該產(chǎn)品廣泛應(yīng)用于肝纖維化模型、肺纖維化模型或皮膚損傷修復(fù)等動(dòng)物實(shí)驗(yàn)研究,為探索HPCⅢ在疾病發(fā)生發(fā)展中的作用或評價(jià)抗纖維化藥物效果提供可靠工具,適用于體外實(shí)驗(yàn)環(huán)境下的濃度測定及動(dòng)態(tài)變化分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
  • 縮寫:
    HPCⅢ
  • 種屬:
    Mus musculus (Mouse)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:100 Average % 97    
    Range % 92-101    
    1:200 Average % 95    
    Range % 90-100    
    1:400 Average % 91    
    Range % 85-95    
    1:800 Average % 102    
    Range % 98-107    
  • 回收率:
    The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-93    
    EDTA plasma (n=4) 84 80-88    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.492 2.343 2.418 2.316    
    500 1.825 1.716 1.771 1.669    
    250 0.947 1.004 0.976 0.874    
    125 0.571 0.582 0.577 0.475    
    62.5 0.359 0.339 0.349 0.247    
    31.2 0.246 0.237 0.242 0.140    
    15.6 0.164 0.171 0.168 0.066    
    0 0.098 0.105 0.102      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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