Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gzms-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
102
Range %
95-106
1:2
Average %
86
Range %
82-92
1:4
Average %
94
Range %
89-98
1:8
Average %
95
Range %
89-99
回收率:
The recovery of mouse Gzms-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
103
96-107
EDTA plasma (n=4)
93
88-97
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
基因功能參考文獻(xiàn):
Tcf1 binding reduced the activity of a Gzmb-associated regulatory element, and this accounted for the reduced Gzmb expression in Tcf1-expressing NK cells. PMID: 28723565
Differential proteomics were used to identify granzyme B substrates in three unrelated bacteria: Escherichia coli, Listeria monocytogenes, and Mycobacteria tuberculosis. Granzyme B cleaves a highly conserved set of proteins in all three bacteria, which function in vital biosynthetic and metabolic pathways that are critical for bacterial survival under diverse environmental conditions. PMID: 29107333
Released granzyme B induces DNA fragmentation in intraepithelial lymphocytes independently of Perforin PMID: 25750028
serglycin plays a critical role in the maturation of dense-core cytotoxic granules in cytotoxic lymphocytes and the trafficking and storage of perforin and granzyme B, whereas granzyme A is unaffected PMID: 26756195
these results suggest a perforin-independent, extracellular role for GzmB in the pathogenesis of cardiac fibrosis PMID: 26610869
data indicate an involvement of granzyme B in the neuroinflammation mediated by Eomes(+) CD4(+) T cells. PMID: 26436530
Data suggest that targeting granzyme B (GzmB) in selected T cell subsets may provide a strategy to control graft-versus-host disease (GVHD). PMID: 26392464
We conclude that GzmbCre can be used under some conditions to investigate gene function in mature and activated natural killer cells. PMID: 25923440
These findings indicate a significant role for GzmB in extracellular matrix degradation that may have implications in many age-related chronic inflammatory diseases. PMID: 25495009
Different natural variants of granzyme B have a profound impact on the immune response to a common and authentic viral pathogen. PMID: 25502180
Visualization of granzyme B-expressing CD8 T cells during primary and secondary immune responses to Listeria monocytogenes. PMID: 25367158
expedites cytotoxic T-lymphocyte diapedesis via basement membrane remodeling PMID: 25526309
CD4(+) natural killer cells potently promote atherosclerosis by granzyme B (and perforin)-dependent apoptosis. PMID: 25398236
Granzyme B has a role in atherosclerotic plaque development. PMID: 24205352
Data indicate that vascular permeability is reduced in Granzyme B (GZMB)-KO mice after delayed-type hypersensitivity -induced inflammation. PMID: 24791744
GzmB is a negative regulator of hematopoietic stem cell function that is induced by stress and chemotherapy in both HSCs and their niches. PMID: 24752302
studies established a pivotal role for NK-cell NKG2D and granzyme B in the pathogenesis of house dust mite-allergic lung disease, and identified novel therapeutic targets for the prevention and treatment of asthma. PMID: 24290277
The apoptotic cell-in-cell death occurred only in internalized immune killer cells expressing granzyme B PMID: 24113190
Granzyme B deficiency exacerbates lung inflammation in mice after acute lung injury. PMID: 23642129
Genetic deletion of granzyme B does not confer resistance to the development of spontaneous diabetes in non-obese diabetic mice. PMID: 23663075
in the Period 1 gene (Per1(-/-) mice), involved in the negative limb of the molecular clock, display significantly altered rhythms of cytokine (eg, interferon-gamma) and cytolytic factors (eg, perforin and granzyme B) in splenic natural killer (NK) cells. PMID: 23402528
granzyme B is dispensable for beta cell destruction in type 1 diabetes, but is required for efficient early activation of cytotxic T lymphocytes. PMID: 22792290
GzmB-mediated, activation-induced cell death in wild-type T8 cells was responsible for their reduced graft-vs-tumor activity. GzmB(-/-) T8 cells had enhanced expansion, skewed toward an effector/memory phenotype, & made more IFN-gamma & Fas ligand. PMID: 23264653
expression by regulatory t-cells controls lung inflammation during acute viral infection PMID: 22236998
There is a role for Granzyme B in age-related skin thinning and frailty. PMID: 21316440
Data suggest that an interdependent relationship between parasite burden and CD8(+) T cells dictates the onset of perforin/GzmB-mediated ECM. PMID: 21525386
During lymphocytic choriomeningitis virus infection, despite persistent similarities in chromatin of CD8-positive memory cells, continuous transcription does not occur at the granzyme B locus. PMID: 21278341
GzmA/B deficiency in filarial infection is associated with a defense-promoting Th2 cytokine and antibody shift, enhanced early inflammatory gene expression, and a trend of reduced alternatively activated macrophage induction. PMID: 21248253
The herpes simplex virus type 1 LAT protects neuron cells from granzyme B-induced apoptosis and CD8 T-cell killing. PMID: 21177822
miR-223 specifically targets the 3' untranslated region of murine GzmB in vitro, indicating that this miRNA may contribute to control of GzmB translation in resting NK cells. PMID: 20935160
Data show that increased activation-induced apoptosis resulted in impaired survival and a decreased clonal burst size of Spi6 KO iNKT cells, which could be corrected by GrB deficiency. PMID: 20543105
Surprisingly, adoptive transfer of granzyme B-deficient T(reg) cells prevented GVHD lethality, suppressed serum cytokine production in vivo, and prevented target organ damage. PMID: 19965675
Pathogenetically relevant immune reactions in proteolipid protein-overexpressing mice are TCR-dependent and mediated by the classical components of CD8+ T-cell cytotoxicity, perforin, and Gzmb. PMID: 20042681
gzmB has a role in caspase-dependent inhibition of mousepox replication PMID: 19838298
In all tumor models examined thus far, granzyme B is not necessary for tumor rejection mediated by the perforin pathway in vivo. PMID: 12847210
Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells PMID: 14985351
Granzyme-B is one of the key mechanisms through which CD4+CD25+ regulatory T cells induce cell contact-mediated suppression. PMID: 15699103
polymorphonuclear leukocytes from mice and humans lack the 3 cytotoxic effector molecules, granzyme A, granzyme B, and perforin, generally associated with natural killer and cytotoxic T lymphocytes PMID: 15998831
suppression of natural killer cell activity induced by whole body hyperthermia could be mediated through the perforin/granzyme pathway PMID: 16236268
In this study, we discuss our recent findings on granzyme B-induced cell death and discuss the potential relevance of this pathway to CL-induced death of viral-infected and transformed cells. PMID: 16405654
absence of either perforin or granzyme B resulted in cell survival in alloreactive CTL-induced pancreatic beta cells PMID: 16436955
GrB plays a critical role in the T cell receptor-induced cell death of Th2 cells PMID: 16901729
Hop may be cleaved by GzmB as an "innocent bystander" during the induction of apoptosis, but it may also act to facilitate apoptosis in concert with other GzmB substrates PMID: 17005566
Granzyme B expression is augmented in activated T cells from Bcl6-/- knockout mice. PMID: 17125145
Varies considerably more in wild populations (2-18 amino acid differences) than between common laboratory strains (12 of 13 were identical). PMID: 17661907
This is the first report of a major distal regulatory element in the control of granzyme B transcription. PMID: 18222115
For the first time, in vivo data implicate granzyme B expression by regulatory T (Treg) lymphocytes in sustaining long-lived graft survival. PMID: 18802078
In vitro protease substrate profiling of the apoptotic serine protease granzyme B was performed resulting in the delineation of more than 800 cleavage sites in 322 human and 282 mouse substrates PMID: 18836177
granzyme C is activated with persistent antigenic stimulation, providing nonredundant backup protection for the host when granzyme B fails. PMID: 19414782
for high levels of transcription to occur a distinct set of histone modifications needs to be established in addition to histone loss at the proximal promoter of gzmB PMID: 19915065