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  4. Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

  • 中文名稱：
    小鼠胰高血糖素樣肽1(GLP-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08118m
  • 樣本類型：
    serum, plasma, tissue homogenates.
  • 檢測(cè)范圍：
    125 pg/ml-8000 pg/ml.
  • 靈敏度：
    31.25 pg/ml
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠胰高血糖素樣肽1(GLP-1)酶聯(lián)免疫試劑盒（CSB-E08118m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿.樣本中的GLP-1含量。GLP-1（胰高血糖素樣肽-1）是一種由腸道L細(xì)胞分泌的激素，能促進(jìn)胰島β細(xì)胞分泌胰島素，抑制胰高血糖素的分泌，并作用于大腦食欲調(diào)節(jié)中樞，抑制食欲，從而降低血糖和體重。其研究機(jī)制涉及調(diào)節(jié)血糖、減重和心血管保護(hù)等多重作用。試劑盒檢測(cè)范圍為125 pg/ml-8000 pg/ml.，適用于科研領(lǐng)域中對(duì)GLP-1表達(dá)水平的定量分析，如糖尿病發(fā)病機(jī)制研究、代謝調(diào)控藥物篩選、腸道激素功能探索等方向，可為體外實(shí)驗(yàn)提供穩(wěn)定可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    N/A
  • 縮寫：
    GLP-1
  • 種屬：
    Mus musculus (Mouse)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    104

    Range %

    98-110

    1:2

    Average %

    95

    Range %

    91-105

    1:4

    Average %

    101

    Range %

    94-108

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of mouse GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    89

    84-94

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    8000

    2.397

    2.334

    2.365

    2.173

    4000

    1.701

    1.667

    1.684

    1.492

    2000

    1.162

    1.098

    1.130

    0.938

    1000

    0.788

    0.819

    0.803

    0.611

    500

    0.572

    0.551

    0.562

    0.370

    250

    0.413

    0.423

    0.418

    0.226

    125

    0.302

    0.294

    0.298

    0.106

    0

    0.189

    0.195

    0.192

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse GLP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled GLP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1：5

產(chǎn)品評(píng)價(jià)： 我用 CSB-E08118m檢測(cè)自發(fā)性T2DM小鼠db/db小鼠，稀釋比例為1：5，稀釋后濃度較低，檢測(cè)出濃度較低。血清較多的話不稀釋最好，實(shí)驗(yàn)結(jié)果準(zhǔn)確！

By 丁老師

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:5

產(chǎn)品評(píng)價(jià)： 使用CSB-E08118m檢測(cè)糖尿病鼠血清的GLP-1指標(biāo)，標(biāo)準(zhǔn)曲線的OD值為：S0（0.0188），S1（0.1317），S2(0.2477),S3(0.3732),S4(0.5668),S5(1.0190),S6(1.6300),S7(2.1775),正常組OD值范圍在0.12-0.15之間，模型組OD值在0.06-0.09之間。產(chǎn)品檢測(cè)相對(duì)穩(wěn)定，兩次重復(fù)間誤差較小。

By 萬(wàn)老師

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