Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠肺表面活性物質(zhì)相關(guān)蛋白A(SP-A)酶聯(lián)免疫試劑盒（CSB-E08685m）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SFTPA1含量。SFTPA1是一個(gè)重要靶點(diǎn)。其它在相關(guān)生理或病理過(guò)程中可能發(fā)揮關(guān)鍵作用。研究機(jī)制上，目前可能聚焦于它在細(xì)胞信號(hào)傳導(dǎo)、生物合成等方面的功能，探索其與疾病發(fā)生發(fā)展的聯(lián)系，以尋找基于該靶點(diǎn)的治療策略。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，尤其適用于動(dòng)物模型研究中的肺功能評(píng)估、呼吸道疾病機(jī)制探索以及藥物干預(yù)效果評(píng)價(jià)等科研場(chǎng)景，為研究者提供穩(wěn)定可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
縮寫：

SFTPA1
Uniprot No.：

P35242
種屬：

Mus musculus (Mouse)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	84
	Range %	80-92
1:2000	Average %	96
	Range %	91-105
1:4000	Average %	98
	Range %	93-103
1:8000	Average %	93
	Range %	86-98

回收率：

The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	93	90-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
蛋白家族：

SFTPA family
數(shù)據(jù)庫(kù)鏈接：

STRING: 10090.ENSMUSP00000022314

UniGene: Mm.46062